Cortical Constriction During Abscission Involves Helices of ESCRT-III-Dependent Filaments

被引：354

作者：

Guizetti, Julien ^{[2
,3
]}

Schermelleh, Lothar ^{[4
]}

Maentler, Jana ^{[5
]}

Maar, Sandra ^{[2
]}

Poser, Ina ^{[5
]}

Leonhardt, Heinrich ^{[4
]}

Mueller-Reichert, Thomas ^{[1
,3
]}

Gerlich, Daniel W. ^{[2
,3
]}

机构：

[1] Tech Univ Dresden, Med Fac Carl Gustav Carus, Med Theoret Ctr, D-01307 Dresden, Germany

[2] Swiss Fed Inst Technol Zurich ETHZ, Dept Biol, Inst Biochem, CH-8093 Zurich, Switzerland

[3] Marine Biol Lab, Woods Hole, MA 02543 USA

[4] Univ Munich, Ctr Integrated Prot Sci Munich, Dept Biol, D-82152 Planegg Martinsried, Germany

[5] Max Planck Inst Mol Cell Biol & Genet, D-01307 Dresden, Germany

来源：

SCIENCE | 2011年 / 331卷 / 6024期

基金：

瑞士国家科学基金会;

关键词：

MEDIATED ABSCISSION; CYTOKINESIS; MIDBODY; MACHINERY; ALIX; SCISSION; SPASTIN; CELLS;

D O I：

10.1126/science.1201847

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

After partitioning of cytoplasmic contents by cleavage furrow ingression, animal cells remain connected by an intercellular bridge, which subsequently splits by abscission. Here, we examined intermediate stages of abscission in human cells by using live imaging, three-dimensional structured illumination microscopy, and electron tomography. We identified helices of 17-nanometer-diameter filaments, which narrowed the cortex of the intercellular bridge to a single stalk. The endosomal sorting complex required for transport (ESCRT)-III co-localized with constriction zones and was required for assembly of 17-nanometer-diameter filaments. Simultaneous spastin-mediated removal of underlying microtubules enabled full constriction at the abscission site. The identification of contractile filament helices at the intercellular bridge has broad implications for the understanding of cell division and of ESCRT-III-mediated fission of large membrane structures.

引用

页码：1616 / 1620

页数：5

共 26 条

[1] The Rab8 GTPase selectively regulates AP-1B-dependent basolateral transport in polarized Madin!Darby canine kidney cells [J].

Ang, AL ;

Fölsch, H ;

Koivisto, UM ;

Pypaert, M ;

Mellman, I .

JOURNAL OF CELL BIOLOGY, 2003, 163 (02) :339-350

[2] Cytokinesis: Placing and making the final cut [J].

Barr, Francis A. ;

Gruneberg, Ulrike .

CELL, 2007, 131 (05) :847-860

[3] Traction forces of cytokinesis measured with optically modified elastic substrata [J].

Burton, K ;

Taylor, DL .

NATURE, 1997, 385 (6615) :450-454

[4] Differential requirements for Alix and ESCRT-III in cytokinesis and HIV-1 release [J].

Carlton, Jez G. ;

Agromayor, Monica ;

Martin-Serrano, Juan .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (30) :10541-10546

[5] Parallels between cytokinesis and retroviral budding: A role for the ESCRT machinery [J].

Carlton, Jez G. ;

Martin-Serrano, Juan .

SCIENCE, 2007, 316 (5833) :1908-1912

[6] Spastin Couples Microtubule Severing to Membrane Traffic in Completion of Cytokinesis and Secretion [J].

Connell, James W. ;

Lindon, Catherine ;

Luzio, J. Paul ;

Reid, Evan .

TRAFFIC, 2009, 10 (01) :42-56

[7] Wound-induced contractile ring: a model for cytokinesis [J].

Darenfed, H ;

Mandato, CA .

BIOCHEMISTRY AND CELL BIOLOGY, 2005, 83 (06) :711-720

[8] Animal cytokinesis: From parts list to mechanisms [J].

Eggert, Ulrike S. ;

Mitchison, Timothy J. ;

Field, Christine M. .

ANNUAL REVIEW OF BIOCHEMISTRY, 2006, 75 :543-566

[9] Structure and disassembly of filaments formed by the ESCRT-III subunit Vps24 [J].

Ghazi-Tabatabai, Sara ;

Saksena, Suraj ;

Short, Judith M. ;

Pobbati, Ajaybabu V. ;

Veprintsev, Dmitry B. ;

Crowther, R. Anthony ;

Emr, Scott D. ;

Egelman, Edward H. ;

Williams, Roger L. .

STRUCTURE, 2008, 16 (09) :1345-1356

[10] Centriolin anchoring of exocyst and SNARE complexes at the midbody is required for secretory-vesicle-mediated abscission [J].

Gromley, A ;

Yeaman, C ;

Rosa, J ;

Redick, S ;

Chen, CT ;

Mirabelle, S ;

Guha, M ;

Sillibourne, J ;

Doxsey, SJ .

CELL, 2005, 123 (01) :75-87

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