Highly efficient conversion of polysialoganglioside to GM1 with Brevibacterium casei as a microbial biocatalyst

被引：8

作者：

Wang, XD ^{[1
]}

Yin, Z ^{[1
]}

Peng, YF ^{[1
]}

Shen, YL ^{[1
]}

Wei, DZ ^{[1
]}

机构：

[1] E China Univ Sci & Technol, Inst Biochem, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

来源：

BIOCATALYSIS AND BIOTRANSFORMATION | 2005年 / 23卷 / 01期

关键词：

biocatalyst; brevibacterium casei; GM1; chromatography;

D O I：

10.1080/10242420500043846

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel bacterium, which can be used to convert polysialoganglioside to GM1 efficiently, was isolated from soil with ganglioside as the sole source of carbon. It was identified tentatively as Brevibacterium casei. In a typical experiment, 0.8 g of highly purified GM1 (93%) was obtained from 1.5 g free GM1 crude ganglioside after chromatographic separation. The overall yield of this procedure was estimated to be more than 50% (w/w).

引用

页码：29 / 32

页数：4

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