Expression of membrane C1q in human monocyte-derived macrophages is developmentally regulated and enhanced by interferon-γ

被引：35

作者：

Kaul, M ^{[1
]}

Loos, M ^{[1
]}

机构：

[1] Univ Mainz, Inst Med Microbiol & Hyg, D-55101 Mainz, Germany

来源：

FEBS LETTERS | 2001年 / 500卷 / 1-2期

关键词：

monocyte/macrophage; differentiation; complement; C1q; phagocytosis;

D O I：

10.1016/S0014-5793(01)02592-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study investigated when during 'in vitro' maturation macrophages (M Phi) express membrane C1q (mC1q), and whether cell activation affects expression and function of mC1q, Although C1q mRNA was repeatedly detected in freshly isolated monocytes using reverse transcriptase-polymerase chain reaction, Clq protein mas observed only in developing M Phi from day 1 to 4 on using immunodetection and flow cytometry, However, the quantity of mC1q and other M Phi membrane proteins differed strikingly in cells from different donors. We report here for the first time that CD14(+) and CD14(-) mC1q-bearing M Phi can develop, and that interferon-gamma increases mC1q display at the cell surface, and mC1q-mediated phagocytosis, (C) 2001 Published by Elsevier Science B,V, on behalf of the Federation of European Biochemical Societies.

引用

页码：91 / 98

页数：8

共 40 条

[1] ENZYME-LINKED IMMUNOSORBENT-ASSAY FOR CLQ IN HUMAN-SERUM BY USE OF MONOCLONAL-ANTIBODIES [J].

ANTES, U ;

HEINZ, HP ;

LOOS, M .

JOURNAL OF IMMUNOLOGICAL METHODS, 1984, 74 (02) :299-306

[2]

BASHAM TY, 1983, J IMMUNOL, V130, P1492

[3] Homozygous C1q deficiency causes glomerulonephritis associated with multiple apoptotic bodies [J].

Botto, M ;

Dell'Agnola, C ;

Bygrave, AE ;

Thompson, EM ;

Cook, HT ;

Petry, F ;

Loos, M ;

Pandolfi, PP ;

Walport, MJ .

NATURE GENETICS, 1998, 19 (01) :56-59

[4] COMPLEMENT COMPONENTS C1Q, C1R/C1S, AND C1INH IN RHEUMATOID-ARTHRITIS - CORRELATION OF IN-SITU HYBRIDIZATION AND NORTHERN BLOT RESULTS WITH FUNCTION AND PROTEIN CONCENTRATION IN SYNOVIUM AND PRIMARY CELL CULTURES [J].

BREITNER, S ;

STORKEL, S ;

REICHEL, W ;

LOOS, M .

ARTHRITIS AND RHEUMATISM, 1995, 38 (04) :492-498

[5] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].

CHOMCZYNSKI, P ;

SACCHI, N .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159

[6] CD16 - DEVELOPMENTALLY REGULATED IGG FC-RECEPTORS ON CULTURED HUMAN-MONOCYTES [J].

CLARKSON, SB ;

ORY, PA .

JOURNAL OF EXPERIMENTAL MEDICINE, 1988, 167 (02) :408-417

[7] T cell-dependent immune response in C1q-deficient mice:: Defective interferon γ production by antigen-specific T cells [J].

Cutler, AJ ;

Botto, M ;

van Essen, D ;

Rivi, R ;

Davies, KA ;

Gray, D ;

Walport, MJ .

JOURNAL OF EXPERIMENTAL MEDICINE, 1998, 187 (11) :1789-1797

[8] ROSETTE ASSAY FOR THE DETERMINATION OF C-1-Q RECEPTOR-BEARING CELLS [J].

GABAY, Y ;

PERLMANN, H ;

PERLMANN, P ;

SOBEL, AT .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1979, 9 (10) :797-801

[9]

GOLAN MD, 1982, J IMMUNOL, V129, P445

[10]

JACK RM, 1994, J IMMUNOL, V153, P262

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