Gene expression profiling to identify genes associated with high-invasiveness in human squamous cell carcinoma with epithelial-to-mesenchymal transition

被引：29

作者：

Higashikawa, Koichiro ^{[1
]}

Yoneda, Shingo ^{[1
]}

Taki, Masayuki ^{[1
]}

Shigeishi, Hideo ^{[1
]}

Ono, Shigehiro ^{[1
]}

Tobiume, Kel ^{[1
]}

Kamata, Nobuyuki ^{[1
]}

机构：

[1] Hiroshima Univ, Grad Sch Biomed Sci, Div Cervicognathostomatol, Dept Oral & Maxillofacial Surg,Minami Ku, Hiroshima 7348553, Japan

来源：

CANCER LETTERS | 2008年 / 264卷 / 02期

关键词：

EMT; snail; squamous cell carcinoma; microarray;

D O I：

10.1016/j.canlet.2008.01.045

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The process of epithelial-to-mesenchymal transition (EMT) involves the acquisition of high-invasiveness by tumor. Snail represses target genes and induces EMT. In this study, we defined the signatures of gene expressions by cDNA microarray analyses in both human squamous cell carcinoma (SCC) cell lines with spontaneous EMT and with Snail-induced EMT, which exhibited high-invasive behavior in vitro. Of the 17,000 cDNA probes, 61 genes were found differentially expressed with >2- or <0.5-fold ratio and shared among the EMT phenotype cell lines, indicating candidates for invasion-associated genes regulated by Snail. Category analysis showed that these genes were mainly classified as development/differentiation, metabolism, apoptosis, angiogenesis and cell adhesion. These data illustrated that Snail regulates various molecular pathways for the establishment of EMT and the acquisition of high-invasiveness in SCC cells, yielding insight into the progression of SCC. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

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页码：256 / 264

页数：9

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