Expressed sequence tags from the midgut and an epithelial cell line of Chironomus tentans:: annotation, bioinformatic classification of unknown transcripts and analysis of expression levels

被引：7

作者：

Arvestad, L

Visa, N

Lundeberg, J

Wieslander, L

Savolainen, P ^{[1
]}

机构：

[1] Albanova Univ Ctr, Royal Inst Technol KTH, Dept Biotechnol, SE-10691 Stockholm, Sweden

[2] Albanova Univ Ctr, Royal Inst Technol KTH, Stockholm Bioinformat Ctr, SE-10691 Stockholm, Sweden

[3] Stockholm Univ, Dept Mol Biol & Funct Genom, S-10691 Stockholm, Sweden

来源：

INSECT MOLECULAR BIOLOGY | 2005年 / 14卷 / 06期

关键词：

Chironomus tentans; midgut; epithelial cell; expressed sequence tag; open reading frame;

D O I：

10.1111/j.1365-2583.2005.00600.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Expressed sequence tags (ESTs) were generated from two Chironomus tentans cDNA libraries, constructed from an embryo epithelial cell line and from larva midgut tissue. 8584 5'-end ESTs were generated and assembled into 3110 tentative unique transcripts, providing the largest contribution of C. tentans sequences to public databases to date. Annotation using BLAST gave 1975 (63.5%) transcripts with a significant match in the major gene/protein databases, 1170 with a best match to Anopheles gambiae and 480 to Drosophila melanogaster. 1091 transcripts (35.1%) had no match to any database. Studies of open reading frames suggest that at least 323 of these contain a coding sequence, indicating that a large proportion of the genes in C. tentans belong to previously unknown gene families.

引用

页码：689 / 695

页数：7

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