A fast and sensitive method for measuring the integrity of siRNA-carrier complexes in full human serum

被引：109

作者：

Buyens, Kevin ^{[1
]}

Lucas, Bart ^{[1
]}

Raemdonck, Koen ^{[1
]}

Braeckmans, Kevin ^{[1
]}

Vercammen, Jo ^{[2
]}

Hendrix, Jelle ^{[2
]}

Engelborghs, Yves ^{[2
]}

De Smedt, Stefaan C. ^{[1
]}

Sanders, Niek N. ^{[1
,3
]}

机构：

[1] Univ Ghent, Fac Pharm, Lab Gen Biochem & Phys Pharm, B-9000 Ghent, Belgium

[2] Univ Louvain, Lab Biomol Dynam, B-3001 Louvain, Belgium

[3] Univ Ghent, Fac Vet Med, Dept Nutr Genet & Ethol, Lab Vet Gene Therapy, B-9820 Merelbeke, Belgium

来源：

JOURNAL OF CONTROLLED RELEASE | 2008年 / 126卷 / 01期

关键词：

siRNA; cationic liposomes; serum; flourescence fluctuation spectroscopy; complex integrity;

D O I：

10.1016/j.jconrel.2007.10.024

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

SiRNA based therapeutics are currently under investigation for treatment of cancer and viral infections. Upon intravenous administration, the nanoscopic delivery systems which carry the siRNA need to be stable in serum, an aspect which is often overlooked in numerous publications on siRNA delivery systems. Techniques currently available for studying the dissociation of siRNA-liposome complexes are time consuming and incompatible with full serum. We therefore developed a fluorescence fluctuation spectroscopy (FFS) based method which allows to monitor the integrity of siRNA-carrier complexes. The method can very rapidly provide quantitative information on the complex integrity in biological media, like full human serum, and at very low siRNA concentrations (similar to 20 nM siRNA). Information on the integrity of intravenously injected siRNA nanoparticles in serum is crucial. Consequently, the FFS method reported in this work may find broad applicability in the field of siRNA-carrier design. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：67 / 76

页数：10

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