Structural and functional studies on different human FABP types

被引：85

作者：

Veerkamp, JH ^{[1
]}

van Moerkerk, HTB ^{[1
]}

Prinsen, CFM ^{[1
]}

van Kuppevelt, TH ^{[1
]}

机构：

[1] Univ Nijmegen, Dept Biochem, NL-6500 HB Nijmegen, Netherlands

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 1999年 / 192卷 / 1-2期

关键词：

fatty acid-binding protein; myelin FABP; 11-dansylamino-undecanoic acid; 1-anilinonaphtalene-8-sulfonic acid; retinoic acid; immunological crossreactivity;

D O I：

10.1023/A:1006866119264

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Interaction of various ligands with recombinant proteins of 5 human FABP types was studied by radiochemical and fluorescence procedures. Liver, heart, intestinal and myelin FABP showed a higher affinity for oleic acid than adipocyte FABP. Intestinal and adipocyte FABP had a relatively high K-d value for arachidonic acid. Liver and intestinal FABP showed high affinity for DAUDA in contrast to the other FABP types. ANS was only well bound by liver and adipocyte FABP. Retinol was not bound by any FABP type, retinoic acid only by adipocyte FABP. Data indicate the importance of both electrostatic and hydrophobic interaction for the ligand-FABP binding. The immunological crossreactivity between six human FABP types including epidermal FABP and their respective antibodies raised in rabbit, chicken and mouse appeared to be low and may suggest heterogeneity of protein surface.

引用

页码：137 / 142

页数：6

共 34 条

[1]

BANASZAK L, 1994, ADV PROTEIN CHEM, V45, P89

[2] HUMAN ADIPOCYTE LIPID-BINDING PROTEIN - PURIFICATION OF THE PROTEIN AND CLONING OF ITS COMPLEMENTARY-DNA [J].

BAXA, CA ;

SHA, RS ;

BUELT, MK ;

SMITH, AJ ;

MATARESE, V ;

CHINANDER, LL ;

BOUNDY, KL ;

BERNLOHR, DA .

BIOCHEMISTRY, 1989, 28 (22) :8683-8690

[3]

BENNETT E, 1994, J NEUROCHEM, V63, P1616

[4]

GLATZ JFC, 1984, J BIOL CHEM, V259, P4295

[5] Cellular fatty acid-binding proteins: Their function and physiological significance [J].

Glatz, JFC ;

vanderVusse, GJ .

PROGRESS IN LIPID RESEARCH, 1996, 35 (03) :243-282

[6] Role of portal region lysine residues in electrostatic interactions between heart fatty acid binding protein and phospholipid membranes [J].

Herr, FM ;

Aronson, J ;

Storch, J .

BIOCHEMISTRY, 1996, 35 (04) :1296-1303

[7] SURFACE LYSINE RESIDUES MODULATE THE COLLISIONAL TRANSFER OF FATTY-ACID FROM ADIPOCYTE FATTY-ACID-BINDING PROTEIN TO MEMBRANES [J].

HERR, FM ;

MATARESE, V ;

BERNLOHR, DA ;

STORCH, J .

BIOCHEMISTRY, 1995, 34 (37) :11840-11845

[8] Fatty acid transfer from liver and intestinal fatty acid-binding proteins to membranes occurs by different mechanisms [J].

Hsu, KT ;

Storch, J .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (23) :13317-13323

[9] ONTOGENIC APPEARANCE OF 3 FATTY-ACID BINDING-PROTEINS IN THE RAT STOMACH [J].

ISEKI, S ;

KANDA, T ;

HITOMI, M ;

ONO, T .

ANATOMICAL RECORD, 1991, 229 (01) :51-60

[10] IMMUNOHISTOCHEMICAL LOCALIZATION OF 2 TYPES OF FATTY-ACID-BINDING PROTEINS IN RAT OVARIES DURING POSTNATAL-DEVELOPMENT AND IN IMMATURE RAT OVARIES TREATED WITH GONADOTROPINS [J].

ISEKI, S ;

AMANO, O ;

FUJII, H ;

KANDA, T ;

ONO, T .

ANATOMICAL RECORD, 1995, 241 (02) :235-243

← 1 2 3 4 →