Transient transfection of mammary epithelial cells with a PEI/DNA/adenovirus system

被引：9

作者：

Bischof, J ^{[1
]}

Vietor, I ^{[1
]}

Cotten, M ^{[1
]}

Huber, LA ^{[1
]}

机构：

[1] Inst Mol Pathol, A-1030 Vienna, Austria

来源：

BIOLOGICAL CHEMISTRY | 1999年 / 380卷 / 02期

关键词：

adenovirus; polarized epithelial cells; polyethylenimine; transfection;

D O I：

10.1515/BC.1999.036

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Studies on epithelial cells often require the transient expression of exogenous proteins in polarized epithelial cells. However, the major limitation of this approach has been the difficulty of obtaining transient gene expression in polarized epithelial cell cultures. We report here on the application of a polyethylenimine (PEI)/DNA/adenovirus system for efficient transient gene expression in mammary epithelial cells. Based on luciferase assay and FACScan analysis the PEI/DNA/adenovirus system is shown to be an effective and simple method for transfecting epithelial cells.

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页码：269 / 273

页数：5

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