SNAT2 silencing prevents the osmotic induction of transport system A and hinders cell recovery from hypertonic stress

被引：32

作者：

Bevilacqua, E ^{[1
]}

Bussolati, O ^{[1
]}

Dall'Asta, V ^{[1
]}

Gaccloli, F ^{[1
]}

Sala, R ^{[1
]}

Gazzola, GC ^{[1
]}

Franchi-Gazzola, R ^{[1
]}

机构：

[1] Univ Parma, Dipartimento Med Sperimentale, Sez Patol Gen & Clin, I-43100 Parma, Italy

来源：

FEBS LETTERS | 2005年 / 579卷 / 16期

关键词：

siRNA; amino acids; glutamine; cell volume;

D O I：

10.1016/j.febslet.2005.05.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Under hypertonic conditions the induction of SLC38A2/SNAT2 leads to the stimulation of transport system A and to the increase in the cell content of amino acids. In hypertonically stressed human fibroblasts transfection with two siRNAs for SNAT2 suppressed the increase in SNAT2 mRNA and the stimulation of system A transport activity. Under the same condition, the expansion of the intracellular amino acid pool was significantly lowered and cell volume recovery markedly delayed. It is concluded that the up-regulation of SNAT2 is essential for the rapid restoration of cell volume after hypertonic stress. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3376 / 3380

页数：5

共 23 条

[1] Osmotic regulation of ATA2 mRNA expression and amino acid transport System A activity [J].

Alfieri, RR ;

Petronini, PG ;

Bonelli, MA ;

Caccamo, AE ;

Cavazzoni, A ;

Borghetti, AF ;

Wheeler, KP .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 283 (01) :174-178

[2]

ALFIERI RR, 2002, J PHYSL, V54, P499

[3] Regulation of gene expression by hypertonicity [J].

Burg, MB ;

Kwon, ED ;

Kultz, D .

ANNUAL REVIEW OF PHYSIOLOGY, 1997, 59 :437-455

[4] THE PREFERENTIAL INTERACTION OF L-THREONINE WITH TRANSPORT-SYSTEM ASC IN CULTURED HUMAN FIBROBLASTS [J].

BUSSOLATI, O ;

ROTOLI, BM ;

LARIS, PC ;

DALLASTA, V ;

GAZZOLA, GC .

BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1070 (02) :305-312

[5] The role of system A for neutral amino acid transport in the regulation of cell volume [J].

Bussolati, O ;

Dall'Asta, V ;

Franchi-Gazzola, R ;

Sala, R ;

Rotoli, BM ;

Visigalli, R ;

Casado, J ;

Lopez-Fontanals, M ;

Pastor-Anglada, M ;

Gazzola, GC .

MOLECULAR MEMBRANE BIOLOGY, 2001, 18 (01) :27-38

[6] Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays [J].

Bustin, SA .

JOURNAL OF MOLECULAR ENDOCRINOLOGY, 2000, 25 (02) :169-193

[7] Amino acids are compatible osmolytes for volume recovery after hypertonic shrinkage in vascular endothelial cells [J].

DalL'Asta, V ;

Bussolati, O ;

Sala, R ;

Parolari, A ;

Alamanni, F ;

Biglioli, P ;

Gazzola, GC .

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1999, 276 (04) :C865-C872

[8] THE TRANSPORT OF L-GLUTAMINE INTO CULTURED HUMAN FIBROBLASTS [J].

DALLASTA, V ;

ROSSI, PA ;

BUSSOLATI, O ;

GUIDOTTI, GG ;

GAZZOLA, GC .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1052 (01) :106-112

[9]

DALLASTA V, 1994, J BIOL CHEM, V269, P10485

[10] Cell cycle delay and apoptosis in response to osmotic stress [J].

Dmitrieva, NI ;

Michea, LF ;

Rocha, GM ;

Burg, MB .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 2001, 130 (03) :411-420

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