Xenopus U3 snoRNA GAC-box A′ and box A sequences play distinct functional roles in rRNA processing

被引:42
作者
Borovjagin, AV [1 ]
Gerbi, SA [1 ]
机构
[1] Brown Univ, Div Biol & Med, Providence, RI 02912 USA
关键词
D O I
10.1128/MCB.21.18.6210-6221.2001
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mutations in the 5 ' portion of Xenopus U3 snoRNA were tested for function in oocytes. The results revealed a new cleavage site (AO) in the 3 ' region of vertebrate external transcribed spacer sequences. In addition, U3 mutagenesis uncoupled cleavage at sites 1 and 2, flanking the 5 ' and 3 ' ends of 18S rRNA, and generated novel intermediates: 19S and 18.5S pre-rRNAs. Furthermore, specific nucleotides in Xenopus U3 snoRNA that are required for cleavages in pre-rRNA were identified: box A is essential for site AO cleavage, the GAC-box A ' region is necessary for site I cleavage, and the 3 ' end of box A ' and flanking nucleotides are required for site 2 cleavage. Differences between metazoan and yeast U3 snoRNA-mediated rRNA processing are enumerated. The data support a model where metazoan U3 snoRNA acts as a bridge to draw together the 5 ' and 3 ' ends of the 18S rRNA coding region within pre-rRNA to coordinate their cleavage.
引用
收藏
页码:6210 / 6221
页数:12
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