Borrelia tanukii sp nov and Borrelia turdae sp nov found from ixodid ticks in Japan: Rapid species identification by 16S rRNA gene-targeted PCR analysis

被引：80

作者：

Fukunaga, M ^{[1
]}

Hamase, A ^{[1
]}

Okada, K ^{[1
]}

Nakao, M ^{[1
]}

机构：

[1] ASAHIKAWA MED COLL,DEPT PARASITOL,ASAHIKAWA,HOKKAIDO 078,JAPAN

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1996年 / 40卷 / 11期

关键词：

Borrelia tanukii; Borrelia turdae; phyla; 16S rRNA gene; PCR;

D O I：

10.1111/j.1348-0421.1996.tb01154.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Based on the results of RFLP-ribotyping, whole DNA/DNA hybridization and phylogenetic analysis of the 16S rRNA gene, we previously defined two genomic groups of spirochetes closely related to Borrelia burgdorferi sensu late: group Hk501 for strains isolated from Ixodes tanuki ticks and group Ya501 for strains isolated from Ixodes turdus ticks. In this report, we propose that group Hk501 should be classified as Borrelia tanukii sp. nov. and group Ya501 as Borrelia turdae sp, nov, The alignment of previously published Borrelia 168 rRNA gene sequences led us to design species-specific PCR primer sets. The primers allowed the rapid identification of B. tanukii and B. turdae.

引用

页码：877 / 881

页数：5

共 26 条

[1] ANTIGENICALLY VARIABLE BORRELIA-BURGDORFERI ISOLATED FROM COTTONTAIL RABBITS AND IXODES-DENTATUS IN RURAL AND URBAN AREAS [J].

ANDERSON, JF ;

MAGNARELLI, LA ;

LEFEBVRE, RB ;

ANDREADIS, TG ;

MCANINCH, JB ;

PERNG, GC ;

JOHNSON, RC .

JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (01) :13-20

[2]

ANDERSON JF, 1989, REV INFECT DIS, V11, pS1451

[3]

ASSOUS MV, 1994, FEMS MICROBIOL LETT, V121, P93, DOI 10.1111/j.1574-6968.1994.tb07081.x

[4] DELINEATION OF BORRELIA-BURGDORFERI SENSU-STRICTO, BORRELIA-GARINII SP-NOV, AND GROUP VS461 ASSOCIATED WITH LYME BORRELIOSIS [J].

BARANTON, G ;

POSTIC, D ;

SAINTGIRONS, I ;

BOERLIN, P ;

PIFFARETTI, JC ;

ASSOUS, M ;

GRIMONT, PAD .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1992, 42 (03) :378-383

[5]

BARBOUR AG, 1984, YALE J BIOL MED, V57, P521

[6] MONOCLONAL-ANTIBODIES FOR IDENTIFICATION OF BORRELIA-AFZELII SP-NOV - ASSOCIATED WITH LATE CUTANEOUS MANIFESTATIONS OF LYME BORRELIOSIS [J].

CANICA, MM ;

NATO, F ;

DUMERLE, L ;

MAZIE, JC ;

BARANTON, G ;

POSTIC, D .

SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES, 1993, 25 (04) :441-448

[7] IXODES TICKS (ACARINA-IXODIDAE) OF NEPAL [J].

CLIFFORD, CM ;

HOOGSTRAAL, H ;

KEIRANS, JE .

JOURNAL OF MEDICAL ENTOMOLOGY, 1975, 12 (01) :115-137

[8] A phylogenetic analysis of Borrelia burgdorferi sensu lato isolates associated with Lyme disease in Japan by flagellin gene sequence determination [J].

Fukunaga, M ;

Koreki, Y .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1996, 46 (02) :416-421

[9] TANDEM REPEAT OF THE 23S AND 5S RIBOSOMAL-RNA GENES IN BORRELIA-BURGDORFERI, THE ETIOLOGIC AGENT OF LYME-DISEASE [J].

FUKUNAGA, M ;

SOHNAKA, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 183 (03) :952-957

[10] Characterization of spirochetes isolated from ticks (Ixodes tanuki, Ixodes turdus, and Ixodes columnae) and comparison of the sequences with those of Borrelia burgdorferi sensu lato strains [J].

Fukunaga, M ;

Hamase, A ;

Okada, H ;

Inoue, H ;

Tsuruta, Y ;

Miyamoto, K ;

Nakao, M .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (07) :2338-2344

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