Improved 1HN-detected triple resonance TROSY-based experiments

被引：139

作者：

Yang, DW

Kay, LE

机构：

[1] Univ Toronto, Prot Engn Network Ctr Excellence, Toronto, ON M5S 1A8, Canada

[2] Univ Toronto, Dept Mol & Med Genet, Toronto, ON M5S 1A8, Canada

[3] Univ Toronto, Dept Biochem, Toronto, ON M5S 1A8, Canada

[4] Univ Toronto, Dept Chem, Toronto, ON M5S 1A8, Canada

来源：

JOURNAL OF BIOMOLECULAR NMR | 1999年 / 13卷 / 01期

基金：

英国医学研究理事会;

关键词：

coherence transfer selection by gradients; triple resonance NMR; TROSY;

D O I：

10.1023/A:1008329230975

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A pulse scheme resulting in improved sensitivity in TROSY-based (HN)-H-1-detected triple resonance experiments is presented. The approach minimizes relaxation losses which occur during the transfer of transverse magnetization from N-15 to (HN)-H-1 immediately prior to detection. The utility of the method is demonstrated on a complex of methyl protonated, highly deuterated maltose binding protein (MBP, 370 residues) and beta-cyclodextrin. Sensitivity gains relative to previous TROSY schemes of approximately 10 and 20% are noted in HNCO spectra of MBP recorded at 25 and 5 degrees C, respectively, corresponding to molecular correlation times of 23 and 46 ns.

引用

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页码：3 / 10

页数：8

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