Postdifferential display: Parallel processing of candidates using small amounts of RNA

被引：12

作者：

Poirier, GMC ^{[1
]}

Erlander, MG

机构：

[1] Univ Birmingham, Sch Med, Dept Anat, Birmingham B15 2TT, W Midlands, England

[2] RW Johnson Pharmaceut Res Inst, San Diego, CA 92121 USA

来源：

METHODS-A COMPANION TO METHODS IN ENZYMOLOGY | 1998年 / 16卷 / 04期

关键词：

D O I：

10.1006/meth.1998.0699

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The necessity of screening differentially expressed candidate genes has imposed a limit on the application of differential display to large-scale analysis of gene expression patterns. screening candidates has indeed proven a burden because traditional screening methods require the purification of large amounts of RNA. In this article we describe an assay that allows the screening of 240 candidate genes with only 5 mu g of total RNA. This assay consists of using cDNA probes synthesized from amplified RNA in differential screening and can be performed in a 96-well plate format. (C) 1998 Academic Press.

引用

页码：444 / 452

页数：9

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