Antiplatelet, Antithrombotic, and Fibrinolytic Activities of Campomanesia xanthocarpa

被引：35

作者：

Klafke, Jonatas Zeni ^{[1
,2
]}

da Silva, Mariane Arnoldi ^{[1
,2
]}

Rossato, Mateus Fortes ^{[1
]}

Trevisan, Gabriela ^{[1
]}

Bandero Walker, Cristiani Isabel ^{[1
]}

Martins Leal, Claudio Alberto ^{[1
]}

Borges, Diego Olschowsky ^{[2
]}

Chitolina Schetinger, Maria Rosa ^{[1
]}

Moresco, Rafael Noal ^{[3
]}

Medeiros Frescura Duarte, Marta Maria ^{[4
]}

Soares dos Santos, Adair Roberto ^{[5
]}

Nazario Viecili, Paulo Ricardo ^{[2
]}

Ferreira, Juliano ^{[1
]}

机构：

[1] Univ Fed Santa Maria, Programa Posgrad Ciencias Biol Bioquim Toxicol, BR-97105900 Santa Maria, RS, Brazil

[2] Univ Cruz Alta, Grp Multidisciplinar Saude, BR-98020290 Cruz Alta, RS, Brazil

[3] Univ Fed Santa Maria, Ctr Ciencias Saude, Programa Posgrad Ciencias Farmaceut, BR-97105900 Santa Maria, RS, Brazil

[4] Univ Luterana Brasil, Dept Ciencias Saude, BR-97020001 Santa Maria, RS, Brazil

[5] Univ Fed Santa Catarina, Dept Physiol Sci, BR-88040970 Florianopolis, SC, Brazil

来源：

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2012年 / 2012卷

关键词：

DIRECT THROMBIN INHIBITOR; BLEEDING-TIME; AGENTS; PLATELETS; DEATH; ASSAY;

D O I：

10.1155/2012/954748

中图分类号：

R [医药、卫生];

学科分类号：

100218 [急诊医学];

摘要：

In a previous work based on popular belief, Campomanesia xanthocarpa Berg., popularly known as "guavirova", showed to have a potential effect in the control of a number of conditions associated with cardiovascular diseases. The aim of the present work was to investigate the effects of C. xanthocarpa extract (CXE) on antiplatelet, antithrombotic and fibrinolytic activities in mice and in human blood. Mice were treated orally for 5 days with CXE or acetylsalicylic acid and at the end of the treatment period animals were challenged for bleeding, acute thromboembolism and ulcerogenic activity. In addition, we have assessed the prothrombin time and activated partial thromboplastin time (aPTT) after oral administration. In in vitro assays, antiplatelet effects of CXE was evaluated on platelet aggregation, and fibrinolytic activity of the extract was observed by mice or human artificial blood clot degradation. Platelet citotoxicity of the extract was also determined by the LDH assay. Results demonstrated that CXE has a significant protective effect on thrombosis. It also inhibits platelet aggregation without demonstrating cytotoxicity on platelets. CXE slightly prolonged aPTT and showed no ulcerogenic activity after oral administration. In addition, CXE showed a fibrinolytic activity. Thus, C. xanthocarpa showed antiplatelet, antithrombotic and fibrinolytic activities in mice.

引用

页数：8

共 43 条

[1]

D-dimer antigen: current concepts and future prospects [J].

Adam, Soheir S. ;

Key, Nigel S. ;

Greenberg, Charles S. .

BLOOD, 2009, 113 (13) :2878-2887

[2]

Alice CB., 1995, PLANTAS MED USO POPU

[3]

Anti-thrombotic effect of chronic oral treatment with Orbignya phalerata Mart. [J].

Azevedo, Ana Paula S. ;

Farias, Jardel C. ;

Costa, Graciomar C. ;

Ferreira, Susanne C. P. ;

Aragao-Filho, Walmir C. ;

Sousa, Paulo R. A. ;

Pinheiro, Mayara T. ;

Maciel, Marcia C. G. ;

Silva, Lucilene A. ;

Lopes, Adelson S. ;

Barroqueiro, Elizabeth S. B. ;

Borges, Marilene O. R. ;

Guerra, Rosane N. M. ;

Nascimento, Flavia R. F. .

JOURNAL OF ETHNOPHARMACOLOGY, 2007, 111 (01) :155-159

[4]

Scientific and therapeutic advances in antiplatelet therapy [J].

Bhatt, DL ;

Topol, EJ .

NATURE REVIEWS DRUG DISCOVERY, 2003, 2 (01) :15-28

[5]

Born G. V. R., 1963, J PHYSL, V95, P168

[6]

DAVIES MJ, 1985, BRIT HEART J, V53, P363

[7]

Antiplatelet drugs [J].

De Meyer, Simon F. ;

Vanhoorelbeke, Karen ;

Broos, Katleen ;

Salles, Isabelle I. ;

Deckmyn, Hans .

BRITISH JOURNAL OF HAEMATOLOGY, 2008, 142 (04) :515-528

[8]

BLEEDING-TIME IN LABORATORY-ANIMALS .2. COMPARISON OF DIFFERENT ASSAY CONDITIONS IN RATS [J].

DEJANA, E ;

CALLIONI, A ;

QUINTANA, A ;

DEGAETANO, G .

THROMBOSIS RESEARCH, 1979, 15 (1-2) :191-197

[9]

Dickel, Michele Luciane ;

Kuze Rates, Stela Maris ;

Ritter, Mara Rejane .

JOURNAL OF ETHNOPHARMACOLOGY, 2007, 109 (01) :60-71

[10]

DIMINNO G, 1983, J PHARMACOL EXP THER, V225, P57

← 1 2 3 4 5 →