Initial electron donor and acceptor in isolated Photosystem II reaction centers identified with femtosecond mid-IR spectroscopy

被引:158
作者
Groot, ML
Pawlowicz, NP
van Wilderen, LJGW
Breton, J
van Stokkum, IHM
van Grondelle, R
机构
[1] Vrije Univ Amsterdam, Fac Sci, NL-1081 HV Amsterdam, Netherlands
[2] CEA Saclay, Serv Bioenerget, F-91191 Gif Sur Yvette, France
关键词
electron transfer; photosynthesis; pump-probe;
D O I
10.1073/pnas.0503483102
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Despite the apparent similarity between the plant Photosystem 11 reaction center (RC) and its purple bacterial counterpart, we show in this work that the mechanism of charge separation is very different for the two photosynthetic RCs. By using femtosecond visible-pump-mid-infrared probe spectroscopy in the region of the chlorophyll ester and keto modes, between 1,775 and 1,585 cm(-1), with 150-fs time resolution, we show that the reduction of pheophytin occurs on a 0.6- to 0.8-ps time scale, whereas P+, the precursor state for water oxidation, is formed after approximate to 6 ps. We conclude therefore that in the Photosystem 11 RC the primary charge separation occurs between the "accessory chlorophyll" ChI(D1) and the pheophytin on the so-called active branch.
引用
收藏
页码:13087 / 13092
页数:6
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