Induction of outward current by orexin-B in mouse peritoneal macrophages

被引：29

作者：

Ichinose, M ^{[1
]}

Asai, M

Sawada, M

Sasaki, K

Oomura, Y

机构：

[1] Shimane Med Univ, Dept Physiol, Izumo, Shimane 6938501, Japan

[2] Shimane Med Univ, Dept Phys, Izumo, Shimane 6938501, Japan

[3] Toyama Univ, Fac Engn, Dept Bioinformat Engn, Toyama 9308555, Japan

[4] Nippon Zoki Pharmaceut Co Ltd, Inst Bioact Sci, Hyogo 6731461, Japan

来源：

FEBS LETTERS | 1998年 / 440卷 / 1-2期

关键词：

orexin; patch-clamp; outward current; mouse macrophage;

D O I：

10.1016/S0014-5793(98)01432-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To define effects of novel feeding regulating peptides, orexins, in immunocompetent cells, ion channel activity in mouse peritoneal macrophages was analyzed by the perforated patch-clamp method. Orexin-B (OX-B) induced an outward current at smaller holding potentials than K+ equilibrium potentials, Reversal potentials of OX-B induced current were dependent on external K+ concentrations but not on external Cl- concentration. Orexin-A is less effective than OX-B, Quinine blocked the outward current and tetraethylammonium partially suppressed the current. These results suggest that OX-B can modulate macrophage functions through the activation of Ca2+-dependent K2+ channels. (C) 1998 Federation of European Biochemical Societies.

引用

页码：51 / 54

页数：4

共 18 条

[1] NYSTATIN PERFORATED-PATCH RECORDING AND ITS APPLICATIONS TO ANALYSES OF INTRACELLULAR MECHANISMS [J].

AKAIKE, N ;

HARATA, N .

JAPANESE JOURNAL OF PHYSIOLOGY, 1994, 44 (05) :433-473

[2] The hypocretins: Hypothalamus-specific peptides with neuroexcitatory activity [J].

De Lecea, L ;

Kilduff, TS ;

Peyron, C ;

Gao, XB ;

Foye, PE ;

Danielson, PE ;

Fukuhara, C ;

Battenberg, ELF ;

Gautvik, VT ;

Bartlett, FS ;

Frankel, WN ;

van den Pol, AN ;

Bloom, FE ;

Gautvik, KM ;

Sutcliffe, JG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (01) :322-327

[3]

DELAFUENTE M, 1991, IMMUNOLOGY, V73, P205

[4] Obesity - The alphabet of weight control [J].

Friedman, JM .

NATURE, 1997, 385 (6612) :119-120

[5] Leptin can induce proliferation, differentiation, and functional activation of hemopoietic cells [J].

Gainsford, T ;

Willson, TA ;

Metcalf, D ;

Handman, E ;

McFarlane, C ;

Ng, A ;

Nicola, NA ;

Alexander, WS ;

Hilton, DJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (25) :14564-14568

[6]

GALLILY R, 1967, IMMUNOLOGY, V12, P197

[7] INTERACTION OF CHEMOTACTIC FACTORS WITH HUMAN MACROPHAGES - INDUCTION OF TRANSMEMBRANE POTENTIAL CHANGES [J].

GALLIN, EK ;

GALLIN, JI .

JOURNAL OF CELL BIOLOGY, 1977, 75 (01) :277-289

[8] ION CHANNELS IN LEUKOCYTES [J].

GALLIN, EK .

PHYSIOLOGICAL REVIEWS, 1991, 71 (03) :775-811

[9] THE ACTIVATION OF CA2+-DEPENDENT K+ CONDUCTANCE BY ADRENALINE IN MOUSE PERITONEAL-MACROPHAGES [J].

HARA, N ;

ICHINOSE, M ;

SAWADA, M ;

MAENO, T .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1991, 419 (3-4) :371-379

[10] ACTIVATION OF K+ CURRENT IN MACROPHAGES BY PLATELET-ACTIVATING-FACTOR [J].

ICHINOSE, M ;

HARA, N ;

SAWADA, M ;

MAENO, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 182 (01) :372-378

← 1 2 →