Insulin-stimulated plasma membrane fusion of Glut4 glucose transporter-containing vesicles is regulated by phospholipase D1

被引:45
作者
Huang, P
Altshuller, YM
Hou, JC
Pessin, JE
Frohman, MA [1 ]
机构
[1] SUNY Stony Brook, Med Ctr, Dept Pharmacol, Stony Brook, NY 11794 USA
[2] SUNY Stony Brook, Med Ctr, Ctr Dev Genet, Stony Brook, NY 11794 USA
关键词
D O I
10.1091/mbc.e04-12-1124
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Insulin stimulates glucose uptake in fat and muscle by mobilizing Glut4 glucose transporters from intracellular membrane storage sites to the plasma membrane. This process requires the trafficking of Glut4-containing vesicles toward the cell periphery, docking at exocytic sites, and plasma membrane fusion. We show here that phospholipase D (PLD) production of the lipid phosphatidic acid (PA) is a key event in the fusion process. PLD1 is found on Glut4-containing vesicles, is activated by insulin signaling, and traffics with Glut4 to exocytic sites. Increasing PLD1 activity facilitates glucose uptake, whereas decreasing PLD1 activity is inhibitory. Diminished PA production does not substantially hinder trafficking of the vesicles or their docking at the plasma membrane, but it does impede fusion-mediated extracellular exposure of the transporter. The fusion block caused by RNA interference-mediated PLD1 deficiency is rescued by exogenous provision of a lipid that promotes fusion pore formation and expansion, suggesting that the step regulated by PA is late in the process of vesicle fusion.
引用
收藏
页码:2614 / 2623
页数:10
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