Construction of a tethered poly(ethylene glycol) surface gradient for studies of cell adhesion kinetics

被引:45
作者
Mougin, K
Ham, AS
Lawrence, MB
Fernandez, EJ
Hillier, AC [1 ]
机构
[1] Iowa State Univ, Dept Chem Engn, Ames, IA 50011 USA
[2] Iowa State Univ, Dept Chem, Ames, IA 50011 USA
[3] Univ Virginia, Dept Chem Engn, Charlottesville, VA 22904 USA
[4] Univ Virginia, Dept Biomed Engn, Charlottesville, VA 22908 USA
关键词
D O I
10.1021/la050613v
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Surface gradients can be used to perform a wide range of functions and represent a novel experimental platform for combinatorial discovery and analysis. In this work, a gradient in the coverage of a surface-immobilized poly(ethylene glycol) (PEG) layer is constructed to interrogate cell adhesion on a solid surface. Variation of surface coverage is achieved by controlled transport of a reactive PEG precursor from a point source through a hydrated gel. Immobilization of PEG is achieved by covalent attachment of the PEG molecule via direct coupling chemistry to a cystamine self-assembled monolayer on gold. This represents a simple method for creating spatial gradients in surface chemistry that does not require special instrumentation or microfabrication procedures. The structure and spatial distribution of the PEG gradient are evaluated via ellipsometry and atomic force microscopy. A cell adhesion assay using bovine arteriole endothelium cells is used to study the influence of PEG thickness and chain density on biocompatibility. The kinetics of cell adhesion are quantified as a function of the thickness of the PEG layer. Results depict a surface in which the variation in layer thickness along the PEG gradient strongly modifies the biological response.
引用
收藏
页码:4809 / 4812
页数:4
相关论文
共 31 条
[1]   Terminal attachment of polyethylene glycol (PEG) chains to a gold electrode surface. Cyclic voltammetry applied to the quantitative characterization of the flexibility of the attached PEG chains and of their penetration by mobile PEG chains [J].
Anne, A ;
Demaille, C ;
Moiroux, J .
MACROMOLECULES, 2002, 35 (14) :5578-5586
[2]   Active spatiotemporal control of electrochemical reactions by coupling to in-plane potential gradients [J].
Balss, KM ;
Coleman, BD ;
Lansford, CH ;
Haasch, RT ;
Bohn, PW .
JOURNAL OF PHYSICAL CHEMISTRY B, 2001, 105 (37) :8970-8978
[3]   HOW TO MAKE WATER RUN UPHILL [J].
CHAUDHURY, MK ;
WHITESIDES, GM .
SCIENCE, 1992, 256 (5063) :1539-1541
[4]   Gradients of substrate-bound laminin orient axonal specification of neurons [J].
Dertinger, SKW ;
Jiang, XY ;
Li, ZY ;
Murthy, VN ;
Whitesides, GM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (20) :12542-12547
[5]  
DUNAWAY DJ, 1994, LANGMUIR, V10, P8
[6]   A WETTABILITY GRADIENT-METHOD FOR STUDIES OF MACROMOLECULAR INTERACTIONS AT THE LIQUID SOLID INTERFACE [J].
ELWING, H ;
WELIN, S ;
ASKENDAL, A ;
NILSSON, U ;
LUNDSTROM, I .
JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1987, 119 (01) :203-210
[7]   PROTEIN AND DETERGENT INTERACTION PHENOMENA ON SOLID-SURFACES WITH GRADIENTS IN CHEMICAL-COMPOSITION [J].
ELWING, H ;
GOLANDER, CG .
ADVANCES IN COLLOID AND INTERFACE SCIENCE, 1990, 32 (04) :317-339
[8]   Microengineering of cellular interactions [J].
Folch, A ;
Toner, M .
ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, 2000, 2 :227-+
[9]   WETTING AND PLASMA-PROTEIN ADSORPTION STUDIES USING SURFACES WITH A HYDROPHOBICITY GRADIENT [J].
GOLANDER, CG ;
LIN, YS ;
HLADY, V ;
ANDRADE, JD .
COLLOIDS AND SURFACES, 1990, 49 (3-4) :289-302
[10]  
HARRIS JM, 1992, POLYETHYLEEN GLYCOL