Simultaneous cell morphometry and refractive index measurement with dual-wavelength digital holographic microscopy and dye-enhanced dispersion of perfusion medium

被引：158

作者：

Rappaz, Benjamin ^{[1
]}

Charriere, Florian ^{[2
]}

Depeursinge, Christian ^{[2
]}

Magistretti, Pierre J. ^{[1
,3
]}

Marquet, Pierre ^{[3
]}

机构：

[1] Ecole Polytech Fed Lausanne, Brain Mind Inst, CH-1015 Lausanne, Switzerland

[2] Ecole Polytech Fed Lausanne, Imaging & Appl Opt Inst, CH-1015 Lausanne, Switzerland

[3] DP CHUV, Ctr Neurosci Psychiat, CH-1008 Prilly, Switzerland

来源：

OPTICS LETTERS | 2008年 / 33卷 / 07期

关键词：

D O I：

10.1364/OL.33.000744

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Digital holographic microscopy (DHM) allows optical-path-difference (OPD) measurements with nanometric accuracy. OPD induced by transparent cells depends on both the refractive index (RI) of cells and their morphology. This Letter presents a dual-wavelength DHM that allows us to separately measure both the RI and the cellular thickness by exploiting an enhanced dispersion of the perfusion medium achieved by the utilization of an extracellular dye. The two wavelengths are chosen in the vicinity of the absorption peak of the dye, where the absorption is accompanied by a significant variation of the RI as a function of the wavelength. (C) 2008 Optical Society of America.

引用

页码：744 / 746

页数：3

共 11 条

[1]

[Anonymous], KRAMERSKRONIG RELATI

[2] VARIATION OF REFRACTIVE INDEX OF SOLUTIONS WITH WAVELENGTH [J].

BARER, R .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1969, 17 (06) :423-&

[3]

CHAUBAL KA, 1967, ACTA HISTOCHEM, V26, P131

[4] Numerical parametric lens for shifting, magnification, and complete aberration compensation in digital holographic microscopy [J].

Colomb, Tristan ;

Montfort, Frederic ;

Kuehn, Jonas ;

Aspert, Nicolas ;

Cuche, Etienne ;

Marian, Anca ;

Charriere, Florian ;

Bourquin, Sebastien ;

Marquet, Pierre ;

Depeursinge, Christian .

JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 2006, 23 (12) :3177-3190

[5] Refractive index measurement in viable cells using quantitative phase-amplitude microscopy and confocal microscopy [J].

Curl, CL ;

Bellair, CJ ;

Harris, T ;

Allman, BE ;

Harris, PJ ;

Stewart, AG ;

Roberts, A ;

Nugent, KA ;

Delbridge, LMD .

CYTOMETRY PART A, 2005, 65A (01) :88-92

[6] Investigation of living pancreas tumor cells by digital holographic microscopy [J].

Kemper, Bjorn ;

Carl, Daniel ;

Schnekenburger, Jurgen ;

Bredebusch, Ilona ;

Schaefer, Marcus ;

Domschke, Wolfram ;

von Bally, Gert .

JOURNAL OF BIOMEDICAL OPTICS, 2006, 11 (03)

[7] Real-time dual-wavelength digital holographic microscopy with a single hologram acquisition [J].

Kuehn, Jonas ;

Colomb, Tristan ;

Montfort, Frederic ;

Charriere, Florian ;

Emery, Yves ;

Cuche, Etienne ;

Marquet, Pierre ;

Depeursinge, Christian .

OPTICS EXPRESS, 2007, 15 (12) :7231-7242

[8] Live cell refractometry using microfluidic devices [J].

Lue, Niyom ;

Popescu, Gabriel ;

Ikeda, Takahiro ;

Dasari, Ramachandra R. ;

Badizadegan, Kamran ;

Feld, Michael S. .

OPTICS LETTERS, 2006, 31 (18) :2759-2761

[9] High-resolution quantitative phase-contrast microscopy by digital holography [J].

Mann, CJ ;

Yu, LF ;

Lo, CM ;

Kim, MK .

OPTICS EXPRESS, 2005, 13 (22) :8693-8698

[10] Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy [J].

Marquet, P ;

Rappaz, B ;

Magistretti, PJ ;

Cuche, E ;

Emery, Y ;

Colomb, T ;

Depeursinge, C .

OPTICS LETTERS, 2005, 30 (05) :468-470

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