Genomic analysis of a new mammalian distal-less gene: DIx7

被引:70
作者
Nakamura, S
Stock, DW
Wydner, KL
Bollekens, JA
Takeshita, K
Nagai, BM
Chiba, S
Kitamura, T
Freeland, TM
Zhao, ZY
Minowada, J
Lawrence, JB
Weiss, KM
Ruddle, FH
机构
[1] YALE UNIV,DEPT BIOL,NEW HAVEN,CT 06511
[2] PENN STATE UNIV,DEPT ANTHROPOL,UNIVERSITY PK,PA 16802
[3] UNIV MASSACHUSETTS,SCH MED,DEPT CELL BIOL,WORCESTER,MA 01655
[4] MT SINAI HOSP,DIV MOL & DEV BIOL,TORONTO,ON M5G 1X5,CANADA
[5] NYU,MED CTR,DEPT MED,NEW YORK,NY 10016
[6] STANFORD UNIV,SCH MED,STANFORD,CA 94305
[7] UNIV TOKYO,FAC MED,DEPT MED 3,TOKYO 113,JAPAN
[8] DNAX RES INST MOL & CELLULAR BIOL INC,PALO ALTO,CA 94304
[9] FUJISAKI CELL CTR,HAYASHIBARA BIOCHEM LABS,OKAYAMA 702,JAPAN
关键词
D O I
10.1006/geno.1996.0634
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We have cloned a new Dlx gene (Dlx7) from human and mouse that may represent the mammalian orthologue of the newt gene NvHBox-5. The homeodomains of these genes are highly similar to all other vertebrate Dlx genes, and regions of similarity also exist between mammalian Dlx7 and a subset of vertebrate Dlx genes downstream of the homeodomain. The sequence divergence between human and mouse Dlx7 in these regions is greater than that predicted from comparisons of other vertebrate Dlx genes, however, and there is little sequence similarity upstream of the homeodomain both between these two genes and with other Dlx genes. We present evidence for alternative splicing of mouse Dlx7 upstream of the homeodomain that may account for some of this divergence. We have mapped human DLX7 distal to the 5' end of the HOXB cluster at an estimated distance of between 1 and 2 Mb by FISH. Both the human and the mouse Dlx7 are shown to be closely linked to Dlx3 in a convergently transcribed orientation. These mapping results support the possibility that vertebrate distal-less genes have been duplicated in concert with the Hox clusters. (C) 1996 Academic Press, Inc.
引用
收藏
页码:314 / 324
页数:11
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