Molecular interactions between poly(ADP-ribose) polymerase (PARP I) and topoisomerase I (Topo I): identification of topology of binding

被引：38

作者：

Bauer, PI

Chen, HJ

Kenesi, E

Kenessey, I

Buki, KG

Kirsten, E

Hakam, A

Hwang, JI

Kun, E ^{[1
]}

机构：

[1] Univ Calif San Francisco, Dept Anat & Cellular & Mol Pharmacol, San Francisco, CA 94143 USA

[2] Semmelweis Univ, Dept Med Biochem, H-1085 Budapest, Hungary

[3] Taipei Univ, Dept Mol Biol, Taipei, Taiwan

来源：

FEBS LETTERS | 2001年 / 506卷 / 03期

基金：

匈牙利科学研究基金会;

关键词：

poly(ADP-ribose) polymerase I; topoisomerase I binding; topoisomerase I regulation by poly(ADP-ribose); polymerase I; binding sited; topology interaction;

D O I：

10.1016/S0014-5793(01)02919-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The molecular interactions of poly(ADP-ribose) polymerase I (PARP I) and topoisomerase I (Topo I) have been determined by the analysis of physical binding of the two proteins and some of their polypeptide components and by the effect of PARP I on the enzymatic catalysis of Topo I. Direct association of Topo I and PARP I as well as the binding of two Topo I polypeptides to PARP I are demonstrated. The effect of PARP I on the 'global' Topo I reaction (scission and religation), and the activation of Topo I by the 36 kDa polypeptide of PARP I and catalytic modifications by poly(ADP-ribosyl)ation are also shown. The covalent binding of Topo I to circular DNA is activated by PARP I similar to the degree of activation of the 'global' Topo I reaction, whereas the religation of DNA is unaffected by PARP I. The geometry of PARP I-Topo I interaction compared to automodified PARP I was reconstructed from direct binding assays between glutathione S-transferase fusion polypeptides of Topo I and PARP I demonstrating highly selective binding, which was correlated with amino acid sequences and with the 'C clamp' model derived from X-ray crystallography. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：239 / 242

页数：4

共 21 条

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[2]

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[3]

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