Gene transfer into mammalian cells using histone-condensed plasmid DNA

被引：139

作者：

Fritz, JD ^{[1
]}

Herweijer, H ^{[1
]}

Zhang, GF ^{[1
]}

Wolff, JA ^{[1
]}

机构：

[1] UNIV WISCONSIN,WAISMAN CTR 355,DEPT PEDIAT & MED GENET,MADISON,WI 53705

来源：

HUMAN GENE THERAPY | 1996年 / 7卷 / 12期

关键词：

D O I：

10.1089/hum.1996.7.12-1395

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A recombinant histone (NLS-H1) containing both the SV40 large T antigen nuclear localization signal and the carboxy-terminal domain of human histone H1 degrees was produced in bacteria, NLS-H1-plasmid DNA complexes, in the presence of chloroquine, mediated reporter gene transfer into cultured cells with similar efficiencies as plasmid DNA-cationic lipid (lipofectin) complexes, NIH-3T3 or COS-7 cells transfected with NLS-H1-plasmid DNA-lipofectin complexes expressed at Least 20 times more luciferase or had at least 2.5 times more beta-galactosidase-positive cells than those transfected with plasmid DNA-lipofectin complexes, Foreign gene expression was also improved by other DNA-binding proteins and cationic lipid formulations, yet the greatest enhancement was obtained with complexes containing either NLS-H1 or calf thymus histone H1, Histone H1-plasmid DNA-lipofectin complexes were internalized by a greater number of cells than plasmid DNA-lipofectin complexes.

引用

页码：1395 / 1404

页数：10

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