Measurement of group delay dispersion of high numerical aperture objective lenses using two-photon excited fluorescence

被引:90
作者
Guild, JB
Xu, C
Webb, WW
机构
[1] School of Applied and Engineering Physics, Cornell University, Ithaca, NY
来源
APPLIED OPTICS | 1997年 / 36卷 / 01期
关键词
D O I
10.1364/AO.36.000397
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We determined the group-delay dispersion (GDD) of five microscope objectives by measuring the second-order autocorrelation at the focal points of the objectives with two-photon excited fluorescence as the power square sensor. We found that typical microscope lens systems introduce significant GDD (2000-6500 fs(2)). The third-order dispersion determined for these objectives limits the minimum obtainable pulse width at the focal point of an objective to 20-30 fs if not compensated. No significant chromatic aberration or higher-order dispersion effects were found for any of the optical components measured within the wavelength range of 700-780 nm and for pulse widths greater than 50-60 fs. (C) 1991 Optical Society of America
引用
收藏
页码:397 / 401
页数:5
相关论文
共 14 条
[1]   2-PHOTON LASER SCANNING FLUORESCENCE MICROSCOPY [J].
DENK, W ;
STRICKLER, JH ;
WEBB, WW .
SCIENCE, 1990, 248 (4951) :73-76
[2]  
Denk Winfried, 1995, P445
[3]   CONTROL AND MEASUREMENT OF ULTRASHORT PULSE SHAPES (IN AMPLITUDE AND PHASE) WITH FEMTOSECOND ACCURACY [J].
DIELS, JCM ;
FONTAINE, JJ ;
MCMICHAEL, IC ;
SIMONI, F .
APPLIED OPTICS, 1985, 24 (09) :1270-1282
[4]   COMPRESSION OF OPTICAL PULSES TO 6 FEMTOSECONDS BY USING CUBIC PHASE COMPENSATION [J].
FORK, RL ;
CRUZ, CHB ;
BECKER, PC ;
SHANK, CV .
OPTICS LETTERS, 1987, 12 (07) :483-485
[5]   NEGATIVE DISPERSION USING PAIRS OF PRISMS [J].
FORK, RL ;
MARTINEZ, OE ;
GORDON, JP .
OPTICS LETTERS, 1984, 9 (05) :150-152
[6]   2-PHOTON EXCITATION OF FLUORESCENCE BY PICOSECOND LIGHT PULSES [J].
GIORDMAINE, JA ;
RENTZEPIS, PM ;
SHAPIRO, SL ;
WECHT, KW .
APPLIED PHYSICS LETTERS, 1967, 11 (07) :216-+
[7]   IMPACT OF CHROMATIC AND SPHERICAL-ABERRATION ON THE FOCUSING OF ULTRASHORT LIGHT-PULSES BY LENSES [J].
KEMPE, M ;
RUDOLPH, W .
OPTICS LETTERS, 1993, 18 (02) :137-139
[8]  
LOUDON R, 1983, QUANTUM THEORY LIGHT, P82
[9]   PULSE DISTORTION IN SINGLE-MODE FIBERS [J].
MARCUSE, D .
APPLIED OPTICS, 1980, 19 (10) :1653-1660
[10]   3-DIMENSIONAL CONFOCAL FLUORESCENCE IMAGING UNDER ULTRASHORT-PULSE ILLUMINATION [J].
GU, M ;
TANNOUS, T ;
SHEPPARD, CJR .
OPTICS COMMUNICATIONS, 1995, 117 (5-6) :406-412