Monitoring of ribosomes and subunits in Escherichia coli during production of glucose isomerase using flow field-flow fractionation

被引：11

作者：

Nilsson, M ^{[1
]}

Wahlund, KG ^{[1
]}

Bulow, L ^{[1
]}

机构：

[1] Univ Lund, Ctr Chem & Chem Engn, SE-22100 Lund, Sweden

来源：

BIOTECHNOLOGY TECHNIQUES | 1998年 / 12卷 / 06期

关键词：

D O I：

10.1023/A:1008867508039

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Ribosomal components and tRNA of E. coli TG1 producing a recombinant enzyme, glucose isomerase, were monitored by asymmetrical flow field-flow fractionation. The growth temperatures were 20, 30 and 37 degrees C. Results indicate a correlation between translational capacity and the amount obtained of the recombinant enzyme. Highest yield was obtained when cultivating at 30 degrees C.

引用

页码：477 / 480

页数：4

共 9 条

[1]

[Anonymous], 1989, SYNTHETIC OLIGONUCLE

[2]

BALBAS P, 1990, METHOD ENZYMOL, V185, P14

[3]

PLASMID PRESENCE CHANGES THE RELATIVE LEVELS OF MANY HOST-CELL PROTEINS AND RIBOSOME COMPONENTS IN RECOMBINANT ESCHERICHIA-COLI [J].