Trichogynes and fertilization in uni- and bimating type colonies of Neurospora tetrasperma

Microscopic examination of living, protoperithecium-bearing colonies of a, A, and a + A that have been challenged by macroconidia from the same three colony types has shown that active trichogynes, i.e., those that grow to and fuse with a conidium, are to be found only in the first two types, Thus, in the a colony the trichogynes respond to conidia from the A and a + A colonies while in the A colony they respond to conidia from a and a + A colonies, In contrast to this ability of conidia from a + A colonies to function as fertilizing elements, the trichogynes of these colonies, if indeed they are formed at all, do not so respond, This nonresponse in a + A colonies may be due to the perithecia that are developing at the time of the challenge, Evidence for this conclusion comes from unimating type colonies in which the two halves of each colony were challenged at different times, 48 h apart, Trichogynes and perithecia developed in the first half; neither developed in the second. This inhibition of trichogyne development and response in the presence of developing perithecia may be only one manifestation of a more general inhibitory action by these structures. (C) 1996 Academic Press, Inc.