Spectroscopic investigation of the structure of a protein adsorbed on a hydrophobic latex

被引：44

作者：

Maste, MCL

Pap, EHW

vanHoek, A

Norde, W

Visser, AJWG

机构：

[1] AGR UNIV WAGENINGEN,DEPT BIOCHEM,WAGENINGEN,NETHERLANDS

[2] AGR UNIV WAGENINGEN,DEPT MOLEC PHYS,WAGENINGEN,NETHERLANDS

来源：

JOURNAL OF COLLOID AND INTERFACE SCIENCE | 1996年 / 180卷 / 02期

关键词：

protein; adsorption; hydrophobic particle; fluorescence; circular dichroism;

D O I：

10.1006/jcis.1996.0348

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Spectroscopic methods provide a powerful tool for investigating the structural properties of immobilized proteins, For that purpose, there was a strong need for a hydrophobic particle that allows determination of the adsorbed protein conformation by fluorescence and circular dichroism. Among the various hydrophobic suspensions that are available, perfluoro-alkoxy fluoro carbon Teflon latex satisfies the requirements of low light absorption and scattering, As an example, preliminary results of structural changes of a proteolytic enzyme dissolved in aqueous solution and in the adsorbed state are given. (C) 1996 Academic Press, Inc.

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收藏

页码：632 / 633

页数：2

相关论文

共 15 条

[1] CRYSTAL-STRUCTURE OF THE ALKALINE PROTEINASE SAVINASE FROM BACILLUS-LENTUS AT 1.4-A RESOLUTION [J].

BETZEL, C ;

KLUPSCH, S ;

PAPENDORF, G ;

HASTRUP, S ;

BRANNER, S ;

WILSON, KS .

JOURNAL OF MOLECULAR BIOLOGY, 1992, 223 (02) :427-445

[2] DRIVING FORCES FOR ENZYME ADSORPTION AT SOLID-LIQUID INTERFACES .1. THE SERINE-PROTEASE SAVINASE [J].

DUINHOVEN, S ;

POORT, R ;

VANDERVOET, G ;

AGTEROF, WGM ;

NORDE, W ;

LYKLEMA, J .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1995, 170 (02) :340-350

[3] CIRCULAR-DICHROISM STUDIES ON CONFORMATIONAL-CHANGES IN PROTEIN MOLECULES UPON ADSORPTION ON ULTRAFINE POLYSTYRENE PARTICLES [J].

KONDO, A ;

MURAKAMI, F ;

HIGASHITANI, K .

BIOTECHNOLOGY AND BIOENGINEERING, 1992, 40 (08) :889-894

[4]

KONDO A, 1993, COLLOID SURFACE B, V1, P197

[5] LIGHT-SCATTERING EFFECTS IN THE MEASUREMENT OF MEMBRANE MICROVISCOSITY WITH DIPHENYLHEXATRIENE [J].

LENTZ, BR ;

MOORE, BM ;

BARROW, DA .

BIOPHYSICAL JOURNAL, 1979, 25 (03) :489-494

[6] INACTIVATION OF A SUBTILISIN IN COLLOIDAL SYSTEMS [J].

MASTE, MCL ;

RINIA, HA ;

BRANDS, CMJ ;

EGMOND, MR ;

NORDE, W .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1995, 1252 (02) :261-268

[7] PROTEIN ADSORPTION AT SOLID LIQUID INTERFACES - REVERSIBILITY AND CONFORMATION ASPECTS [J].

NORDE, W ;

MACRITCHIE, F ;

NOWICKA, G ;

LYKLEMA, J .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1986, 112 (02) :447-456

[8] STRUCTURE OF ADSORBED AND DESORBED PROTEINS [J].

NORDE, W ;

FAVIER, JP .

COLLOIDS AND SURFACES, 1992, 64 (01) :87-93

[9]

NORDE W, 1991, Journal of Biomaterials Science Polymer Edition, V2, P183

[10] QUANTITATION OF THE INTERACTION OF PROTEIN-KINASE-C WITH DIACYLGLYCEROL AND PHOSPHOINOSITIDES BY TIME-RESOLVED DETECTION OF RESONANCE ENERGY-TRANSFER [J].

PAP, EHW ;

BASTIAENS, PIH ;

BORST, JW ;

VANDENBERG, PAW ;

VANHOEK, A ;

SNOEK, GT ;

WIRTZ, KWA ;

VISSER, AJWG .

BIOCHEMISTRY, 1993, 32 (48) :13310-13317