E-dispar strain:: Analysis of polymorphism as a tool for study of geographic distribution

被引:23
作者
Ramos, F
García, G
Valadez, A
Morán, P
González, E
Gómez, A
Melendro, EI
Valenzuela, O
Ximénez, C
机构
[1] Natl Autonomous Univ Mexico, Fac Med, Dept Expt Med, Mexico City 06726, DF, Mexico
[2] Inst Mexicano Seguro Social, Coodinac Invest Salud, Mexico City 06726, DF, Mexico
关键词
E; dispar; histolytica; intra-species polymorphism; chitinase gene; geographic mobility;
D O I
10.1016/j.molbiopara.2005.02.010
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The intra-species polymorphism of E. histolytica and E. dispar species in endemic area is an important tool for geographic distribution and spread mechanism studies. Since E. dispar and E. histolytica shears ecological niche, cell cycle, and transmission mechanism for human host, we studied the intra-specie variation and distribution of E. dispar strains obtained from cyst passers, in two neighbor rural communities in Morelos Mexico. We analyzed the polymorphic region of the quitinase protein gene in isolates of E. dispar. In 45 isolates from one community we identified 12 different CHI patterns while in 15 isolates from the other community we identified 5 different patterns. However both communities share 4 patterns. This finding suggests the presence of strains with different geographic mobility. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:175 / 177
页数:3
相关论文
共 9 条
[1]   ENTAMOEBA-HISTOLYTICA EXTRACHROMOSOMAL CIRCULAR RIBOSOMAL DNA - ANALYSIS OF CLONAL VARIATION IN A HYPERVARIABLE REGION [J].
BHATTACHARYA, S ;
BHATTACHARYA, A ;
DIAMOND, LS .
EXPERIMENTAL PARASITOLOGY, 1992, 74 (02) :200-204
[2]   ENTAMOEBA-HISTOLYTICA - A METHOD FOR ISOLATE IDENTIFICATION [J].
CLARK, CG ;
DIAMOND, LS .
EXPERIMENTAL PARASITOLOGY, 1993, 77 (04) :450-455
[3]   AXENIC CULTIVATION OF ENTAMOEBA-DISPAR BRUMPT 1925, ENTAMOEBA-INSOLITA GEIMAN AND WICHTERMAN 1937 AND ENTAMOEBA-RANARUM GRASSI 1879 [J].
CLARK, CG .
JOURNAL OF EUKARYOTIC MICROBIOLOGY, 1995, 42 (05) :590-593
[4]  
CLARK CG, 1993, DIAGNOSTIC MOL MICRO, V4, P468
[5]   MULTIPLE SEQUENCE ALIGNMENT WITH HIERARCHICAL-CLUSTERING [J].
CORPET, F .
NUCLEIC ACIDS RESEARCH, 1988, 16 (22) :10881-10890
[6]   Cloning and expression of chitinases of Entamoebae [J].
delaVega, H ;
Specht, CA ;
Semino, CE ;
Robbins, PW ;
Eichinger, D ;
Caplivski, D ;
Ghosh, S ;
Samuelson, J .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1997, 85 (02) :139-147
[7]   Molecular epidemiology of Entamoeba spp.:: Evidence of a bottleneck (Demographic sweep) and transcontinental spread of diploid parasites [J].
Ghosh, S ;
Frisardi, M ;
Ramirez-Avila, L ;
Descoteaux, S ;
Sturm-Ramirez, K ;
Newton-Sanchez, OA ;
Santos-Preciado, JI ;
Ganguly, C ;
Lohia, A ;
Reed, S ;
Samuelson, J .
JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (10) :3815-3821
[8]   Remarkable genetic polymorphism among Entamoeba histolytica isolates from a limited geographic area [J].
Haghighi, A ;
Kobayashi, S ;
Takeuchi, T ;
Masuda, G ;
Nozaki, T .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (11) :4081-4090