Membrane lipid perturbation modifies the set point of the temperature of heat shock response in yeast

被引:163
作者
Carratu, L
Franceschelli, S
Pardini, CL
Kobayashi, GS
Horvath, I
Vigh, L
Maresca, B
机构
[1] CNR,INT INST GENET & BIOPHYS,I-80125 NAPLES,ITALY
[2] ST LOUIS UNIV,MED CTR,DEPT INTERNAL MED,ST LOUIS,MO 63110
[3] HUNGARIAN ACAD SCI,BIOL RES CTR,H-6701 SZEGED,HUNGARY
关键词
Delta(9)-desaturase; membrane physical state;
D O I
10.1073/pnas.93.9.3870
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 [理学]; 0710 [生物学]; 09 [农学];
摘要
Addition of a saturated fatty acid (SEA) induced a strong increase in heat shock (HS) mRNA transcription when cells were heat-shocked at 37 degrees C, whereas treatment with an unsaturated fatty acid (UFA) reduced or eliminated the level of HS gene transcription at 37 degrees C. Transcription of the Delta(9)-desaturase gene (Ole1) of Histoplasma capsulatum, whose gene product is responsible for the synthesis of UFA, is up-regulated in a temperature-sensitive strain. We show that when the L8-14C mutant of Saccharomyces cerevisiae, which has a disrupted Ole1 gene, is complemented with its own Ole1 coding region under control of its own promoter or Ole1 promoters of H. capsulatum, the level of EIS gene transcription depends on the activity of the promoters. Fluorescence anisotropy, of mitochondrial membranes of complemented strains corresponded to the different activity of the Ole1 promoter used. We propose that the SFA/UFA ratio and perturbation of membrane lipoprotein complexes are involved in the perception of rapid temperature changes and under HS conditions disturbance of the preexisting membrane physical state causes transduction of a signal that induces transcription of HS genes.
引用
收藏
页码:3870 / 3875
页数:6
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