Interactions of heterologous DNA with polyomavirus major structural protein, VPI

被引：30

作者：

Stokrová, J

Palková, Z

Fischer, L

Richterová, Z

Korb, J

Griffin, BE

Forstová, J

机构：

[1] Charles Univ, Fac Nat Sci, CR-12844 Prague 2, Czech Republic

[2] Acad Sci Czech Republ, Inst Mol Genet, CR-16637 Prague 6, Czech Republic

[3] Univ London Imperial Coll Sci Technol & Med, Hammersmith Hosp, Sch Med, London W12 0NN, England

来源：

FEBS LETTERS | 1999年 / 445卷 / 01期

关键词：

capsid-like particle; DNA-protein interaction; DNA encapsidation; gene therapy; polyoma virus;

D O I：

10.1016/S0014-5793(99)00003-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

'Empty' polyomavirus pseudocapsids, self-assembled from the major structural protein VP1, bind DNA nonspecifically and can deliver it into the nuclei of mammalian cells for expression [Forstova et al, (1995) Hum. Gene Ther, 6, 297-306], Formation of suitable VP1-DNA complexes appears to be the limiting step in this route of gene delivery. Here, the character of VP1-DNA interactions has been studied in detail. Electron microscopy revealed that VP1 pseudocapsids can create in vitro at least two types of interactions with double-stranded DNA: (i) highly stable complexes, requiring free DNA ends, where the DNA is partially encapsidated; and, (il) weaker interactions of pseudocapsids with internal parts of the DNA chain. (C) 1999 Federation of European Biochemical Societies.

引用

页码：119 / 125

页数：7

共 30 条

[1] CELL-FREE ASSEMBLY OF A POLYOMA-LIKE PARTICLE FROM EMPTY CAPSIDS AND DNA [J].