G&T-seq: parallel sequencing of single-cell genomes and transcriptomes

被引：535

作者：

Macaulay, Iain C. ^{[1
]}

Haerty, Wilfried ^{[2
]}

Kumar, Parveen ^{[3
]}

Li, Yang I. ^{[2
]}

Hu, Tim Xiaoming ^{[2
]}

Teng, Mabel J. ^{[4
]}

Goolam, Mubeen ^{[5
]}

Saurat, Nathalie ^{[6
]}

Coupland, Paul ^{[7
]}

Shirley, Lesley M. ^{[7
]}

Smith, Miriam ^{[7
]}

Van der Aa, Niels ^{[3
]}

Banerjee, Ruby ^{[8
]}

Ellis, Peter D. ^{[7
]}

Quail, Michael A. ^{[7
]}

Swerdlow, Harold P. ^{[7
]}

Zernicka-Goetz, Magdalena ^{[5
]}

Livesey, Frederick J. ^{[6
]}

Ponting, Chris P. ^{[1
,2
]}

Voet, Thierry ^{[1
,3
]}

机构：

[1] Wellcome Trust Sanger Inst, Sanger Inst, EBI Single Cell Genom Ctr, Hinxton, England

[2] Univ Oxford, Dept Physiol Anat & Genet, MRC Funct Genom Unit, Oxford, England

[3] Katholieke Univ Leuven, Dept Human Genet, Leuven, Belgium

[4] Wellcome Trust Sanger Inst, Canc Genome Project, Cambridge, England

[5] Univ Cambridge, Dept Physiol Dev & Neurosci, Cambridge, England

[6] Univ Cambridge, Wellcome Trust Canc Res UK Gurdon Inst, Cambridge, England

[7] Wellcome Trust Sanger Inst, Sequencing R&D, Cambridge, England

[8] Wellcome Trust Sanger Inst, Cytogenet Core Facil, Cambridge, England

来源：

NATURE METHODS | 2015年 / 12卷 / 06期

基金：

英国医学研究理事会; 英国惠康基金; 英国生物技术与生命科学研究理事会;

关键词：

PLURIPOTENT STEM-CELLS; COPY-NUMBER VARIATION; RNA-SEQ; GENE; ALIGNMENT; TUMOR; IDENTIFICATION; INHIBITOR;

D O I：

10.1038/nmeth.3370

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone.

引用

页码：519 / +

页数：7

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