Membrane Topology and Functional Analysis of Methylobacillus sp 12S Genes epsF and epsG, Encoding Polysaccharide Chain-Length Determining Proteins

被引：6

作者：

Ayabe-Chujo, Yuko ^{[1
]}

Usami, Yusuke ^{[1
]}

Yoshida, Takako ^{[1
]}

Omori, Toshio ^{[2
]}

Nojiri, Hideaki ^{[1
]}

机构：

[1] Univ Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo 1138657, Japan

[2] Shibaura Inst Technol, Koto Ku, Tokyo 1358548, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2012年 / 76卷 / 03期

关键词：

extracellular polysaccharide; chain-length; membrane topology; Methylobacillus; phosphorylation; GROUP-1 CAPSULAR POLYSACCHARIDES; TYROSINE-KINASE WZC; SP STRAIN 12S; ESCHERICHIA-COLI; ATPASE ACTIVITY; STREPTOCOCCUS-PNEUMONIAE; EXOP PROTEIN; DOMAIN; PHOSPHORYLATION; SUCCINOGLYCAN;

D O I：

10.1271/bbb.110831

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The EpsF and EpsG of the methanol-assimilating bacterium Methylobacillus sp. 12S are involved in the synthesis of a high molecular weight exopolysaccharide, methanolan. These proteins share homology with chain-length determiners in other polysaccharide-producing bacteria. The N- and C-termini of EpsF were found to locate to the cytoplasm, and EpsF was predicted to have two transmembrane regions. EpsG showed both ATPase and autophosphorylation activities.

引用

页码：608 / 612

页数：5

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