Ectopic bone formation by 3D porous calcium phosphate-Ti6Al4V hybrids produced by perfusion electrodeposition

被引：84

作者：

Chai, Yoke Chin ^{[2
,3
,4
]}

Kerckhofs, Greet ^{[1
,4
]}

Roberts, Scott J. ^{[2
,4
]}

Van Bael, Simon ^{[4
,5
,6
]}

Schepers, Evert ^{[4
,7
]}

Vleugels, Jozef ^{[1
]}

Luyten, Frank P. ^{[2
,4
]}

Schrooten, Jan ^{[1
,4
]}

机构：

[1] Katholieke Univ Leuven, Dept Met & Mat Engn, B-3001 Louvain, Belgium

[2] Katholieke Univ Leuven, Lab Skeletal Dev & Joint Disorders, B-3000 Louvain, Belgium

[3] Univ Malaya, Fac Engn, Dept Biomed Engn, Kuala Lumpur 50603, Malaysia

[4] Katholieke Univ Leuven, Div Skeletal Tissue, B-3001 Louvain, Belgium

[5] Katholieke Univ Leuven, Div Biomech & Engn Design, B-3001 Heverlee, Belgium

[6] Katholieke Univ Leuven, Div Prod Engn Machine Design & Automat, Dept Mech Engn, B-3001 Louvain, Belgium

[7] Katholieke Univ Leuven, Dept Prosthet Dent, B-3000 Louvain, Belgium

来源：

BIOMATERIALS | 2012年 / 33卷 / 16期

关键词：

Bone formation; Calcium phosphate; Osteoinduction; Electrodeposition; Titanium scaffolds; DIAMOND CONCEPT; TITANIUM-ALLOY; CELLS; DIFFERENTIATION; COATINGS; HYDROXYAPATITE; OSTEOINDUCTION; BIOMATERIALS; CERAMICS;

D O I：

10.1016/j.biomaterials.2012.02.026

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Successful clinical repair of non-healing skeletal defects requires the use of bone substitutes with robust bone inductivity and excellent biomechanical stability. Thus, three-dimensionally functionalised porous calcium phosphate-Ti6Al4V (Cap-Ti) hybrids were produced by perfusion electrodeposition, and the in vitro and in vivo biological performances were evaluated using human periosteum derived cells (hPDCs). By applying various current densities at the optimised deposition conditions, CaP coatings with sub-micrometer to nano-scale porous crystalline structures and different ion dissolution kinetics were deposited on the porous Ti6Al4V scaffolds. These distinctive physicochemical properties caused a significant impact on in vitro proliferation, osteogenic differentiation, and matrix mineralisation of hPDCs. This includes a potential role of hPDCs in mediating osteoclastogenesis for the resorption of CaP coatings, as indicated by a significant down-regulation of osteoprotegerin (OPG) gene expression and by the histological observation of abundant multi-nucleated giant cells near to the coatings. By subcutaneous implantation, the produced hybrids induced ectopic bone formation, which was highly dependent on the physicochemical properties of the CaP coating (including the Ca2+ dissolution kinetics and coating surface topography), in a cell density-dependent manner. This study provided further insight on stem cell-Cap biomaterial interactions, and the feasibility to produced bone reparative units that are predictively osteoinductive in vivo by perfusion electrodeposition technology. (c) 2012 Elsevier Ltd. All rights reserved.

引用

页码：4044 / 4058

页数：15

共 32 条

[1]

An electrodeposition method of calcium phosphate coatings on titanium alloy [J].