MRI detection of thrombin with aptamer functionalized superparamagnetic iron oxide nanoparticles

被引：153

作者：

Yigit, Mehmet Veysel ^{[2
,3
]}

Mazumdar, Debapriya ^{[1
,3
]}

Lu, Yi ^{[1
,2
,3
,4
]}

机构：

[1] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

[2] Univ Illinois, Ctr Biophys & Computat Biol, Urbana, IL 61801 USA

[3] Univ Illinois, Beckman Inst Adv Sci & Technol, Urbana, IL 61801 USA

[4] Univ Illinois, Dept Biochem, Urbana, IL 61801 USA

来源：

BIOCONJUGATE CHEMISTRY | 2008年 / 19卷 / 02期

关键词：

D O I：

10.1021/bc7003928

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Desion of smart MRI contrast agent based on superparamagnetic iron oxide nanoparticles and aptamers has been described for the detection of human cc-thrombin protein. The contrast agent is based on the assembly of the aptamer functionalized nanoparticles in the presence of thrombin. A detectable change in MRI signal is observed with 25 nM thrombin in human serum. Changes were neither observed with control analytes, streptavidin, or bovine serum albumin, nor with inactive aptamer functionalized nanoparticles.

引用

页码：412 / 417

页数：6

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