Mechanisms of resistance to Qol fungicides in phytopathogenic fungi

被引:229
作者
Fernandez-Ortuno, Dolores [2 ]
Tores, Juan A. [2 ]
De Vicente, Antonio [1 ]
Perez-Garcia, Alejandro [1 ]
机构
[1] Univ Malaga, Fac Ciencias, Dept Microbiol, CSIC,Grp Microbiol & Plant Pathol, E-29071 Malaga, Spain
[2] CSIC, Expt Stn La Mayora, Malaga, Spain
关键词
alternative respiration; cytochrome b; efflux transporters; fungicide resistance; strobilurins;
D O I
10.2436/20.1501.01.38
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The major threat to crops posed by fungal diseases results in the use by growers of enormous amounts of chemicals. Of these, quinol oxydation inhibitors (QoIs) are probably the most successful class of agricultural fungicides. QoIs inhibit mitochondrial respiration in fungi by binding to the Qo site of the cytochrome bc(1), complex, blocking electron transfer and halting ATP synthesis. Unfortunately, the rapid development of resistance to these fungicides and consequent control failure has become increasingly problematic. The main mechanism conferring resistance to QoIs is target site modification, involving mutations in the cytochrome b gene CYTB, such as the substitution of glycine by alanine at position 143 (G143A) that occurs in several phytopathogenic fungi. The impact of other mechanisms, including alternative respiration and efflux transporters, on resistance seems to be limited. Interestingly, in some species QoI resistance is not supported by mutations in CYTB, while in others the structure of the gene is such that it is unlikely to undergo G143A mutations. Better understanding of the biological basis of QoI resistance in a single pathogen species will facilitate the development of resistance diagnostic tools as well as proper anti-resistance strategies aimed at maintaining the high efficacy of these fungicides.
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页码:1 / 9
页数:9
相关论文
共 39 条
[1]   The ABC transporter AtrB from Aspergillus nidulans mediates resistance to all major classes of fungicides and some natural toxic compounds [J].
Andrade, AC ;
Del Sorbo, G ;
Van Nistelrooy, JGM ;
De Waard, MA .
MICROBIOLOGY-SGM, 2000, 146 :1987-1997
[2]   STROBILURINS - NEW ANTIFUNGAL ANTIBIOTICS FROM BASIDIOMYCETE STROBILURUS-TENACELLUS (PERS EX FR) SING [J].
ANKE, T ;
OBERWINKLER, F ;
STEGLICH, W ;
SCHRAMM, G .
JOURNAL OF ANTIBIOTICS, 1977, 30 (10) :806-810
[3]   Characterization of spontaneous mutants of Magnaporthe grisea expressing stable resistance to the Qo-inhibiting fungicide azoxystrobin [J].
Avila-Adame, C ;
Köller, W .
CURRENT GENETICS, 2003, 42 (06) :332-338
[4]   Review of strobilurin fungicide chemicals [J].
Balba, Hamdy .
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES, 2007, 42 (04) :441-451
[5]   The strobilurin fungicides [J].
Bartlett, DW ;
Clough, JM ;
Godwin, JR ;
Hall, AA ;
Hamer, M ;
Parr-Dobrzanski, B .
PEST MANAGEMENT SCIENCE, 2002, 58 (07) :649-662
[6]   Mitochondrial DNA repair pathways [J].
Bohr, VA ;
Anson, RM .
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 1999, 31 (04) :391-398
[7]   Microscopic and transcriptome analyses of early colonization of tomato roots by Trichoderma harzianum [J].
Chacon, Mariola R. ;
Rodriguez-Galan, Olga ;
Benitez, Tahia ;
Sousa, Sonia ;
Rey, Manuel ;
Llobell, Antonio ;
Delgado-Jarana, Jesus .
INTERNATIONAL MICROBIOLOGY, 2007, 10 (01) :19-27
[8]   Characterizing resistance risk of Erysiphe graminis f.sp tritici to strobilurins [J].
Chin, KM ;
Chavaillaz, D ;
Kaesbohrer, M ;
Staub, T ;
Felsenstein, FG .
CROP PROTECTION, 2001, 20 (02) :87-96
[9]   Impact of fungal drug transporters on fungicide sensitivity, multidrug resistance and virulence [J].
de Waard, MA ;
Andrade, AC ;
Hayashi, K ;
Schoonbeek, HJ ;
Stergiopoulos, I ;
Zwiers, LH .
PEST MANAGEMENT SCIENCE, 2006, 62 (03) :195-207
[10]   Fungal transporters involved in efflux of natural toxic compounds and fungicides [J].
Del Sorbo, G ;
Schoonbeek, HJ ;
De Waard, MA .
FUNGAL GENETICS AND BIOLOGY, 2000, 30 (01) :1-15