A photoinducible 1,3-dipolar cycloaddition reaction for rapid, selective modification of tetrazole-containing proteins

被引：334

作者：

Song, Wenjiao ^{[1
]}

Wang, Yizhong ^{[1
]}

Qu, Jun ^{[2
]}

Madden, Michael M. ^{[1
]}

Lin, Qing ^{[1
]}

机构：

[1] SUNY Buffalo, Dept Chem, Buffalo, NY 14260 USA

[2] SUNY Buffalo, Dept Pharmaceut Sci, Buffalo, NY 14260 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 15期

关键词：

alkenes; biotechnology; dipolar cycloaddition; photolysis; proteins;

D O I：

10.1002/anie.200705805

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Reactive but biologically inert: The bioorthogonal title reaction enables highly specific chemical modifications, such as lipidation, of engineered proteins containing a diphenyltetrazole group (see scheme). The cycloaddition in biological media is extremely fast (≤1 min) and tolerant of proteinaceous groups. Strongly fluorescent pyrazoline cycloadducts are generated with simple alkenes. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

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页码：2832 / 2835

页数：4

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