Temporal profile of Src, SSeCKS, and angiogenic factors after focal cerebral ischemia: Correlations with angiogenesis and cerebral edema

被引：69

作者：

Zan, Likun ^{[1
]}

Wu, He ^{[1
,2
]}

Jiang, Jie ^{[1
]}

Zhao, Shiguang ^{[3
]}

Song, Yuejia ^{[4
]}

Teng, Guoxin ^{[5
]}

Li, Heng ^{[1
]}

Jia, Ying ^{[1
]}

Zhou, Min ^{[1
]}

Zhang, Xin ^{[6
]}

Qi, Jiping ^{[1
]}

Wang, Jian ^{[2
]}

机构：

[1] Harbin Med Univ, Dept Pathol, Affiliated Hosp 1, Harbin 150001, Peoples R China

[2] Johns Hopkins Univ, Dept Anesthesiol Crit Care Med, Sch Med, Baltimore, MD 21205 USA

[3] Harbin Med Univ, Dept Neurosurg, Affiliated Hosp 1, Harbin 150001, Peoples R China

[4] Harbin Med Univ, Dept Digest Syst, Affiliated Hosp 1, Harbin 150001, Peoples R China

[5] Jinan Lixia Peoples Hosp, Dept Pathol, Jinan 250014, Peoples R China

[6] Shanxi Canc Hosp, Dept Colorectal Surg, Taiyuan 030013, Peoples R China

来源：

NEUROCHEMISTRY INTERNATIONAL | 2011年 / 58卷 / 08期

关键词：

Src; SSeCKS; Angiogenesis; Cerebral edema; Ischemia; ENDOTHELIAL GROWTH-FACTOR; CELL-SURVIVAL; MICROVASCULAR HYPERPERMEABILITY; ARTERY OCCLUSION; EXPRESSION; VEGF; ANGIOPOIETIN-2; PERMEABILITY; MECHANISMS; KINASES;

D O I：

10.1016/j.neuint.2011.02.014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

A better understanding of the underlying mechanisms of angiogenesis and vascular permeability is necessary for the development of therapeutic strategies for ischemic injury. The purpose of this study was to examine the spatial and temporal expression of Src and Src-suppressed C kinase substrate (SSeCKS) in brain after middle cerebral artery occlusion (MCAO) and elucidate the relationships among Src, SSeCKS, and the key angiogenic factors present after stroke. Rats were subjected to either MCAO or sham operation. Reverse transcriptase-polymerase chain reaction and Western blotting results revealed that Src gradually increased starting as early as 2 h after MCAO and remained high for 1 day. In contrast, SSeCKS decreased after MCAO. Src expression correlated positively with that of vascular endothelial growth factor and angiopoietin-2, and negatively with that of SSeCKS, angiopoietin-1, and zonula occludens-1. However, SSeCKS had the reverse correlations. Changes in the expression of these factors correlated with the progress of angiogenesis and cerebral edema. Dynamic temporal changes in Src and SSeCKS expression may modulate angiogenesis and cerebral edema formation after focal cerebral ischemia. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：872 / 879

页数：8

共 39 条

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Src family kinase inhibitor PP1 reduces secondary damage after spinal cord compression in rats [J].