Role of the zinc fingers of HIV-1 nucleocapsid protein in maturation of genomic RNA

被引：20

作者：

Baba, S

Takahashi, K

Koyanagi, Y

Yamamoto, N

Takaku, H

Gorelick, RJ

Kawai, G

机构：

[1] Chiba Inst Technol, Fac Engn, Dept Ind Chem, Narashino, Chiba 2750016, Japan

[2] Tohoku Univ, Sch Med, Dept Virol, Aoba Ku, Sendai, Miyagi 9808575, Japan

[3] Tokyo Med & Dent Univ, Fac Med, Dept Mol Virol, Tokyo 1138519, Japan

[4] NCI, SAIC Frederick Inc, dAIDS Vaccine Program, Frederick, MD 21702 USA

来源：

JOURNAL OF BIOCHEMISTRY | 2003年 / 134卷 / 05期

关键词：

DIS; HIV-1; maturation; nucleocapsid protein; zinc finger;

D O I：

10.1093/jb/mvg200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The nucleocapsid protein of HIV-1 consists of two basic amino acid regions and two zinc fingers. We investigated the requirement of these domains for the structural conversion of a 39mer RNA covering the dimerization initiation site by using three peptides; wild-type NCp7, a mutant in which the two zinc fingers are mutated, and another mutant in which the two zinc fingers are deleted. The two mutants exhibited similar conversion activities to each other, which were lower than that of the wildtype, indicating that the two basic regions exhibit some activity for RNA chaperone, as we suggested before, and the zinc fingers enhance the efficiency of this activity.

引用

页码：637 / 639

页数：3

共 18 条

[1] NMR structure of the HIV-1 nucleocapsid protein bound to stem-loop SL2 of the Ψ-RNA packaging signal.: Implications for genome recognition [J].