Graphene-based dental adhesive with anti-biofilm activity

被引：83

作者：

Bregnocchi, Agnese ^{[1
,2
,3
]}

Zanni, Elena ^{[1
,2
,4
]}

Uccelletti, Daniela ^{[1
,2
,4
]}

Marra, Fabrizio ^{[1
,2
,3
]}

Cavallini, Domenico ^{[1
,2
,3
]}

De Angelis, Francesca ^{[5
]}

De Bellis, Giovanni ^{[1
,2
,3
]}

Bossu, Maurizio ^{[5
]}

Ierardo, Gaetano ^{[5
]}

Polimeni, Antonella ^{[5
]}

Sarto, Maria Sabrina ^{[1
,2
,3
]}

机构：

[1] Sapienza Univ Rome, Res Ctr Nanotechnol Appl Engn, Sapienza Univ CNIS, Piazzale Aldo Moro 5, I-00185 Rome, Italy

[2] Sapienza Univ Rome, Sapienza Nanotechnol & Nanosci Lab SNN Lab, Piazzale Aldo Moro 5, I-00185 Rome, Italy

[3] Sapienza Univ Rome, Dept Aerosp Elect & Energy Engn, Via Eudossiana 18, I-00184 Rome, Italy

[4] Sapienza Univ Rome, Dept Biol & Biotechnol Charles Darwin, Piazzale Aldo Moro 5, I-00185 Rome, Italy

[5] Sapienza Univ Rome, Unit Pediat Dent, Dept Dent & Maxillofacial Sci, Viale Regina Elena 287a, I-00161 Rome, Italy

来源：

JOURNAL OF NANOBIOTECHNOLOGY | 2017年 / 15卷

关键词：

ANTIBACTERIAL ACTIVITY; ANTIMICROBIAL PROPERTIES; GRAPHITE NANOPLATELETS; CALCIUM-PHOSPHATE; OXIDATIVE STRESS; BOND STRENGTH; RESIN; OXIDE; CYTOTOXICITY; COMPOSITES;

D O I：

10.1186/s12951-017-0322-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Background: Secondary caries are considered the main cause of dental restoration failure. In this context, antibiofilm and bactericidal properties are desired in dental materials against pathogens such as Streptococcus mutans. To this purpose, graphene based materials can be used as fillers of polymer dental adhesives. In this work, we investigated the possibility to use as filler of dental adhesives, graphene nanoplatelets (GNP), a non toxic hydrophobic nanomaterial with antimicrobial and anti-biofilm properties. Results: Graphene nanoplatelets have been produced starting from graphite intercalated compounds through a process consisting of thermal expansion and liquid exfoliation. Then, a dental adhesive filled with GNPs at different volume fractions has been produced through a solvent evaporation method. The rheological properties of the new experimental adhesives have been assessed experimentally. The adhesive properties have been tested using microtensile bond strength measurements (mu-TBS). Biocidal activity has been studied using the colony forming units count (CFU) method. The anti-biofilm properties have been demonstrated through FE-SEM imaging of the biofilm development after 3 and 24 h of growth. Conclusions: A significantly lower vitality of S. mutans cells has been demonstrated when in contact with the GNP filled dental adhesives. Biofilm growth on adhesive-covered dentine tissues demonstrated anti-adhesion properties of the produced materials. mu-TBS results demonstrated no significant difference in mu-TBS between the experimental and the control adhesive. The rheology tests highlighted the necessity to avoid low shear rate regimes during adhesive processing and application in clinical protocol, and confirmed that the adhesive containing the 0.2% wt of GNPs possess mechanical properties comparable with the ones of the control adhesive.

引用

页数：13

共 60 条

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