High-performance liquid chromatography of some estrogens on chemically bonded phases

被引：9

作者：

Petrovic, SM

Acanski, M

Pejanovic, V

Petrovic, JA

机构：

[1] ICN GALENIKA INST, YU-11000 BELGRADE, YUGOSLAVIA

[2] UNIV NOVI SAD, FAC SCI, INST CHEM, YU-21000 NOVI SAD, YUGOSLAVIA

来源：

CHROMATOGRAPHIA | 1996年 / 43卷 / 9-10期

关键词：

column liquid chromatography; chemically bonded silica gel; mixed solvents; steroids;

D O I：

10.1007/BF02293009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The chromatographic behaviour of 30 estradiol and estrone derivatives has been studied on diol, amino- and cyanopropyl silica gel stationary phases with nonaqueous mobile phases. The slopes of linear relationships between the logarithm of the retention factor and logarithm of the volume fractions of the polar components of the binary eluents have been calculated and are discussed in relation to the characteristics of the solute, eluent and stationary phase. An equation for testing the separation efficiency of stationary phases has been derived.

引用

页码：551 / 556

页数：6

共 7 条

[1] CHARACTERIZATION OF AMINO-ALKYL, CYANO-ALKYL BONDED SILICA COLUMNS IN NORMAL-PHASE LIQUID-CHROMATOGRAPHY BY USING STEROIDS [J].