Enzymatic incorporation of an antibody-activated blue fluorophore into DNA

被引：23

作者：

Kaufmann, GF

Meijler, MM

Sun, CZ

Chen, DW

Kujawa, DP

Mee, JM

Hoffman, TZ

Wirsching, P

Lerner, RA

Janda, KD

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 14期

关键词：

antibodies; DNA recognition; fluorescent probes; nucleotides; polymerase chain reaction;

D O I：

10.1002/anie.200461143

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) Antibodies as light switches: The deoxy-nucleotide analogue-stilbene conjugate 1 can be incorporated into nascent DNA by DNA polymerase activity. The blue fluorescence of stilbene is detected only upon binding of an antibody that specifically recognizes the stilbene-modified nucleotide. Therefore, DNA generated through PCR with 1 in the substrate pool can be used for hybridization assays in which detection is triggered by the antibody (see scheme). © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：2144 / 2148

页数：5

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