Hyaluronan reduces migration and proliferation in CHO cells

被引：22

作者：

Dübe, B

Lüke, HJ

Aumailley, M

Prehm, P

机构：

[1] Univ Munster, Inst Physiol Chem & Pathobiochem, D-48129 Munster, Germany

[2] Inst Biochem, D-50931 Cologne, Germany

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 2001年 / 1538卷 / 2-3期

关键词：

hyaluronan; migration; proliferation; adhesion;

D O I：

10.1016/S0167-4889(01)00079-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Expression of the hyaluronan synthase gene in hyaluronan-deficient CI-IO cells changed the cell morphology from a spindle shape to a flattened epithelial-type form. Hyaluronan producing CHO cells showed reduced initial cell adhesion, migration, proliferation and density at contact inhibition, but no difference in random migration determined by the Boyden chamber assay. Addition of hyaluronan to the medium of CHO cells reduced migration, proliferation and initial cell adhesion. In contrast, coating the plastic dish with hyaluronan enhanced initial cell adhesion. These results are discussed in the contest of the perplexing properties of hyaluronan on cellular functions. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：283 / 289

页数：7

共 25 条

[1] INCREASED HYALURONATE SYNTHESIS IS REQUIRED FOR FIBROBLAST DETACHMENT AND MITOSIS [J].