Alteration of cyclin D1 and CDK4 gene in carcinoma of uterine cervix

被引：60

作者：

Cheung, TH ^{[1
]}

Yu, MMY

Lo, KWK

Yim, SF

Chung, TKH

Wong, YF

机构：

[1] Chinese Univ Hong Kong, Prince Wales Hosp, Dept Obstet & Gynaecol, Kowloon, Hong Kong, Peoples R China

[2] Chinese Univ Hong Kong, Prince Wales Hosp, Dept Anat & Cellular Pathol, Hong Kong, Peoples R China

来源：

CANCER LETTERS | 2001年 / 166卷 / 02期

关键词：

cervical carcinoma; retinoblastoma gene; cyclin D1; CDK4; prognosis;

D O I：

10.1016/S0304-3835(01)00457-8

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Amplification and overexpression of cyclin D1 and CDK4 genes in cervical carcinoma were studied by semi-quantitative differential polymerase chain reaction assay and an immunostaining technique, respectively. Amplifications of cyclin D1 and CDK4 genes were found in 24% (27/113) and 26% (29/112) of tumors, respectively. Overexpression of cyclin D1 and CDK4 was demonstrated in 32% (21/66) and 73% (45/62) of tumors, respectively. No tumor showed CDK4 gene mutation on single strand conformational polymorphism. Sixteen percent (8/49) of the tumor specimens showed neither amplification nor over expression. Disease stage, tumor grade and overexpression of cyclin D1 were found to be independent poor prognostic factors in cervical carcinoma. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：199 / 206

页数：8

共 32 条

[1] Gene amplification and overexpression of CDK4 in sporadic breast carcinomas is associated with high tumor cell proliferation [J].