Smoking cessation and bronchial epithelial remodelling in COPD: a cross-sectional study

被引：86

作者：

Lapperre, Therese S. ^{[1
]}

Sont, Jacob K. ^{[2
]}

van Schadewijk, Annemarie ^{[1
]}

Gosman, Margot M. E. ^{[5
]}

Postma, Dirkje S. ^{[5
]}

Bajema, Ingeborg M. ^{[3
]}

Timens, Wim ^{[6
]}

Mauad, Thais ^{[1
,4
]}

Hiemstra, Pieter S. ^{[1
]}

Kauffman, H. F. ^{[8
]}

de Reus, D. ^{[8
]}

Boezen, H. M. ^{[9
]}

Jansen, D. F. ^{[9
]}

Vonk, J. ^{[9
]}

Barentsen, M. D. W. ^{[10
]}

Timens, W. ^{[10
]}

Zeinstra-Smit, M. ^{[10
]}

Luteijn, A. J. ^{[11
]}

van der Molen, T. ^{[11
]}

ter Veen, G. ^{[11
]}

Gosman, M. M. E. ^{[12
]}

ten Hacken, N. H. T. ^{[12
]}

Kerstjens, H. A. M. ^{[12
]}

van Maaren, M. S. ^{[12
]}

Postma, D. S. ^{[12
]}

Veltman, C. A. ^{[12
]}

Verbokkem, A. ^{[12
]}

Verhage, I. ^{[12
]}

Vink-Kloosters, H. K. ^{[12
]}

Snoeck-Stroband, J. B. ^{[13
]}

Thiadens, H. ^{[13
]}

Sont, J. K. ^{[14
]}

Bajema, I. ^{[15
]}

Gast-Strookman, J. ^{[16
]}

Hiemstra, P. S. ^{[16
]}

Janssen, K. ^{[16
]}

Lapperre, T. S. ^{[16
]}

Rabe, K. F. ^{[16
]}

van Schadewijk, A. ^{[16
]}

Schrumpf, J. A. ^{[16
]}

Smit-Bakker, J. ^{[16
]}

Stolk, J. ^{[16
]}

Tire, A. C. J. A. ^{[16
]}

van der Veen, H. ^{[16
]}

Wijffels, M. M. E. ^{[16
]}

Willems, L. N. A. ^{[16
]}

Sterk, P. J. ^{[17
]}

Mauad, T. ^{[18
]}

机构：

[1] Leiden Univ, Med Ctr, Dept Pulmonol, Leiden, Netherlands

[2] Leiden Univ, Med Ctr, Dept Med Decis Making, Leiden, Netherlands

[3] Leiden Univ, Med Ctr, Dept Pathol, Leiden, Netherlands

[4] Univ Sao Paulo, Dept Pathol, Sao Paulo, Brazil

[5] Univ Groningen, Univ Med Ctr Groningen, Dept Pulmonol, Groningen, Netherlands

[6] Univ Med Ctr Groningen, Dept Pathol, Groningen, Netherlands

[7] Groningen Leiden Univ, Leiden, Netherlands

[8] Univ Med Ctr Groningen, Dept Allergol, Groningen, Netherlands

[9] Univ Med Ctr Groningen, Dept Epidemiol & Stat, Groningen, Netherlands

[10] Univ Med Ctr Groningen, Dept Pathol, Groningen, Netherlands

[11] Univ Med Ctr Groningen, Dept Gen Practice, Groningen, Netherlands

[12] Univ Med Ctr Groningen, Dept Pulmonol, Groningen, Netherlands

[13] Leiden Univ, Med Ctr, Dept Gen Practice, Leiden, Netherlands

[14] Leiden Univ, Med Ctr, Dept Med Decis Making, Leiden, Netherlands

[15] Leiden Univ, Med Ctr, Dept Pathol, Leiden, Netherlands

[16] Leiden Univ, Med Ctr, Dept Pulmonol, Leiden, Netherlands

[17] Univ Amsterdam, Acad Med Ctr, Dept Pulmonol, NL-1012 WX Amsterdam, Netherlands

[18] Univ Sao Paulo, Sao Paulo, Brazil

来源：

RESPIRATORY RESEARCH | 2007年 / 8卷

关键词：

GROWTH-FACTOR RECEPTOR; OBSTRUCTIVE PULMONARY-DISEASE; CHRONIC MUCUS HYPERSECRETION; HUMAN NEUTROPHIL DEFENSINS; AIRWAY INFLAMMATION; PERIPHERAL AIRWAYS; CURRENT SMOKERS; EXPRESSION; INTERVENTION; BRONCHODILATOR;

D O I：

10.1186/1465-9921-8-85

中图分类号：

R56 [呼吸系及胸部疾病];

学科分类号：

摘要：

Background: Chronic Obstructive Pulmonary Disease ( COPD) is associated with bronchial epithelial changes, including squamous cell metaplasia and goblet cell hyperplasia. These features are partially attributed to activation of the epidermal growth factor receptor ( EGFR). Whereas smoking cessation reduces respiratory symptoms and lung function decline in COPD, inflammation persists. We determined epithelial proliferation and composition in bronchial biopsies from current and ex-smokers with COPD, and its relation to duration of smoking cessation. Methods: 114 COPD patients were studied cross-sectionally: 99 males/15 females, age 62 +/- 8 years, median 42 pack-years, no corticosteroids, current (n = 72) or ex-smokers ( n = 42, median cessation duration 3.5 years), postbronchodilator FEV 63 +/- 9% predicted. Squamous cell metaplasia (%), goblet cell ( PAS/Alcian Blue(+)) area (%), proliferating (Ki- 67(+)) cell numbers (/mm basement membrane), and EGFR expression (%) were measured in intact epithelium of bronchial biopsies. Results: Ex-smokers with COPD had significantly less epithelial squamous cell metaplasia, proliferating cell numbers, and a trend towards reduced goblet cell area than current smokers with COPD ( p = 0.025, p = 0.001, p = 0.081, respectively), but no significant difference in EGFR expression. Epithelial features were not different between short-term quitters (<3.5 years) and current smokers. Long-term quitters (= 3.5 years) had less goblet cell area than both current smokers and short-term quitters ( medians: 7.9% vs. 14.4%, p = 0.005; 7.9% vs. 13.5%, p = 0.008; respectively), and less proliferating cell numbers than current smokers (2.8% vs. 18.6%, p < 0.001). Conclusion: Ex-smokers with COPD had less bronchial epithelial remodelling than current smokers, which was only observed after long-term smoking cessation (>3.5 years).

引用

页数：9

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