A novel sensitive and selective bioassay for human type I interferons

被引：58

作者：

Files, JG ^{[1
]}

Gray, JL ^{[1
]}

Do, LT ^{[1
]}

Foley, WP ^{[1
]}

Gabe, JD ^{[1
]}

Nestaas, E ^{[1
]}

Pungor, E ^{[1
]}

机构：

[1] Berlex Labs Inc, Richmond, CA 94804 USA

来源：

JOURNAL OF INTERFERON AND CYTOKINE RESEARCH | 1998年 / 18卷 / 12期

关键词：

D O I：

10.1089/jir.1998.18.1019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a novel MxA gene-induction assay for type I interferons (IFN-alpha and IFN-beta) based on the specific induction of the MxA gene in cultured human cells. Accumulated intracellular MxA protein is determined by immunologic measurement by a rapid method using commercially available materials. IFN activity can be measured accurately over a concentration range of 0.130 IU/ml. In contrast, type II IFN and other cytokines are not significantly detected. The MxA-induction assay has advantages in terms of specificity, reliability, and sensitivity over other methods for assaying type I IFN. It has also been adapted and validated for measuring the titers of anti-IFN-beta neutralizing antibodies in human sera.

引用

页码：1019 / 1024

页数：6

共 25 条

[21] MXA-DEPENDENT INHIBITION OF MEASLES-VIRUS GLYCOPROTEIN-SYNTHESIS IN A STABLY TRANSFECTED HUMAN MONOCYTIC CELL-LINE [J].