Bromopyrogallol red enhanced resonance light-scattering spectroscopic determination of DNA with 5,10,15,20-tetrakis[4-(trimethylammoniumyl)phenyl]porphine

被引：34

作者：

Guo, ZX ^{[1
]}

Li, L ^{[1
]}

Shen, HX ^{[1
]}

Cong, X ^{[1
]}

机构：

[1] Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 1999年 / 379卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

resonance light-scattering spectroscopy; calf thymus DNA; bromopyrogallol red; synergetic effect; 5,10,15,20-tetrakis[4(trimethylammoniumyl)phenyl]porphine;

D O I：

10.1016/S0003-2670(98)00651-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

At pH 7.5 and ion strength 0.004, the intensity of resonance light-scattering (RLS) of 5,10,15,20-tetrakis[4-(trimethylammoniumyl)phenyl]prophine (TAPP) is enhanced by DNA at 434 nm. When bromopyrogallol red (BPR) coexists with DNA and TAPP, the RLS intensity of TAPP is synergetically enhanced by BPR and DNA. Based on this, a highly sensitive method for the determination of DNA was proposed with a linear range for calf thymus DNA from 2.5 x 10(-8) to 9.0 x 10(-7) mol l(-1). The method utilizes an ordinary spectrofluorometer, and has been applied to the analysis of some synthetic samples with good recoveries. The reaction mechanism is also discussed. (C) 1999 Published by Elsevier Science B.V.

引用

页码：45 / 51

页数：7

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