Locus effects and sources of error in noninvasive genotyping

被引:73
作者
Buchan, JC
Archie, EA
Van Horn, RC
Moss, CJ
Alberts, SC
机构
[1] Duke Univ, Dept Biol, Durham, NC 27708 USA
[2] Amboseli Elephant Res Project, Nairobi, Kenya
来源
MOLECULAR ECOLOGY NOTES | 2005年 / 5卷 / 03期
关键词
allelic dropout; faecal DNA; noninvasive genotyping; polymerase slippage; contamination;
D O I
10.1111/j.1471-8286.2005.01002.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In spite of more than a decade of research on noninvasive genetic sampling, the low quality and quantity of DNA in noninvasive studies continue to plague researchers. Effects of locus size on error have been documented but are still poorly understood. Further, sources of error other than allelic dropout have been described but are often not well quantified. Here we analyse the effects of locus size on allelic dropout, amplification success and error rates in noninvasive genotyping studies of three species, and quantify error other than allelic dropout.
引用
收藏
页码:680 / 683
页数:4
相关论文
共 10 条
[1]   Characterization of tetranucleotide microsatellite loci in the African Savannah Elephant (Loxodonta africana africana) [J].
Archie, EA ;
Moss, CJ ;
Alberts, SC .
MOLECULAR ECOLOGY NOTES, 2003, 3 (02) :244-246
[2]   True paternal care in a multi-male primate society [J].
Buchan, JC ;
Alberts, SC ;
Silk, JB ;
Altmann, J .
NATURE, 2003, 425 (6954) :179-181
[3]  
Miller CR, 2002, GENETICS, V160, P357
[4]   Quantitative polymerase chain reaction analysis of DNA from noninvasive samples for accurate microsatellite genotyping of wild chimpanzees (Pan troglodytes verus) [J].
Morin, PA ;
Chambers, KE ;
Boesch, C ;
Vigilant, L .
MOLECULAR ECOLOGY, 2001, 10 (07) :1835-1844
[5]  
NAVIDI W, 1992, AM J HUM GENET, V50, P347
[6]  
Nyakaana S, 1998, MOL ECOL, V7, P1436
[7]   Standardizing for microsatellite length in comparisons of genetic diversity [J].
Petit, RJ ;
Deguilloux, MF ;
Chat, J ;
Grivet, D ;
Garnier-Géré, P ;
Vendramin, GG .
MOLECULAR ECOLOGY, 2005, 14 (03) :885-890
[8]  
Sefc KM, 2003, AUK, V120, P982, DOI 10.1642/0004-8038(2003)120[0982:MAFMFS]2.0.CO
[9]  
2
[10]   Reliable genotyping of samples with very low DNA quantities using PCR [J].
Taberlet, P ;
Griffin, S ;
Goossens, B ;
Questiau, S ;
Manceau, V ;
Escaravage, N ;
Waits, LP ;
Bouvet, J .
NUCLEIC ACIDS RESEARCH, 1996, 24 (16) :3189-3194