PCR-based detection of sterol demethylation inhibitor-resistant strains of Penicillium digitatum

被引：26

作者：

Hamamoto, H ^{[1
]}

Hasegawa, K

Nakaune, R

Lee, YJ

Akutsu, K

Hibi, T

机构：

[1] Univ Tokyo, Dept Agr & Environm Biol, Plant Pathol Lab, Bunkyo Ku, Tokyo 1138657, Japan

[2] Natl Inst Fruit Tree Sci, Akitsu, Hiroshima 7292494, Japan

[3] Ibaraki Univ, Fac Agr, Ami, Ibaraki 3000393, Japan

来源：

PEST MANAGEMENT SCIENCE | 2001年 / 57卷 / 09期

关键词：

spore PCR; demethylation inhibitor fungicide resistance; Penicillium digitatum;

D O I：

10.1002/ps.370

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 [作物学];

摘要：

A simple method for detecting sterol demethylation inhibitor (DMI)-resistant strains of the citrus green mould pathogen, Penicillium digitatum, has been developed. The method involves detection of a tandem repeat of a transcriptional enhancer in the promoter region of PdCYP51, which encodes the target enzyme of DMIs, by PCR, using conidia as template. The presence of the tandem repeat leads to overexpression of this gene and confers DMI resistance to the fungus. We examined the relationship between the presence of the tandem repeat and DMI resistance in 39 strains of P digitatum. The results suggested that the DMI resistance mechanism based on the tandem repeat is common in this fungus. Using this method, the presence of DMI resistance of this fungus can be detected within 5-6 h. (C) 2001 Society of Chemical Industry.

引用

页码：839 / 843

页数：5

共 20 条

[1]

ADACHI K, 1995, THESIS U TOKYO TOKYO

[2]

Brent, 1995, FUNGICIDE RESISTANCE

[3]

UPTAKE OF IMAZALIL BY CITRUS-FRUIT AFTER POSTHARVEST APPLICATION AND THE EFFECT OF RESIDUE DISTRIBUTION ON SPORULATION OF PENICILLIUM-DIGITATUM [J].