Measuring serotonin distribution in live cells with three-photon excitation

被引：376

作者：

Maiti, S ^{[1
]}

Shear, JB ^{[1
]}

Williams, RM ^{[1
]}

Zipfel, WR ^{[1
]}

Webb, WW ^{[1
]}

机构：

[1] CORNELL UNIV, SCH APPL & ENGN PHYS, ITHACA, NY 14853 USA

来源：

SCIENCE | 1997年 / 275卷 / 5299期

关键词：

D O I：

10.1126/science.275.5299.530

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Tryptophan and serotonin were imaged with infrared illumination by three-photon excitation (3PE) of their native ultraviolet (UV) fluorescence. This technique, established by 3PE cross section measurements of tryptophan and the monoamines serotonin and dopamine, circumvents the limitations imposed by photodamage, scattering, and indiscriminate background encountered in other UV microscopies. Three-dimensionally resolved images are presented along with measurements of the serotonin concentration (similar to 50 mM) and content (up to similar to 5 X 10(8) molecules) of individual secretory granutes.

引用

页码：530 / 532

页数：3

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