Cell-free Formation of RNA Granules: Bound RNAs Identify Features and Components of Cellular Assemblies

被引：628

作者：

Han, Tina W. ^{[1
]}

Kato, Masato ^{[1
]}

Xie, Shanhai ^{[1
]}

Wu, Leeju C. ^{[1
]}

Mirzaei, Hamid ^{[1
]}

Pei, Jimin ^{[3
]}

Chen, Min ^{[2
]}

Xie, Yang ^{[2
]}

Allen, Jeffrey ^{[2
]}

Xiao, Guanghua ^{[2
]}

McKnight, Steven L. ^{[1
]}

机构：

[1] Univ Texas SW Med Ctr Dallas, Dept Biochem, Dallas, TX 75390 USA

[2] Univ Texas SW Med Ctr Dallas, Dept Clin Sci, Dallas, TX 75390 USA

[3] Univ Texas SW Med Ctr Dallas, Howard Hughes Med Inst, Dallas, TX 75390 USA

来源：

CELL | 2012年 / 149卷 / 04期

关键词：

AMYOTROPHIC-LATERAL-SCLEROSIS; MESSENGER-RNA; INTRACELLULAR-LOCALIZATION; DENDRITIC LOCALIZATION; BINDING PROTEINS; AMPA RECEPTOR; APLYSIA CPEB; DROSOPHILA; NEURONS; PUMILIO;

D O I：

10.1016/j.cell.2012.04.016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cellular granules lacking boundary membranes harbor RNAs and their associated proteins and play diverse roles controlling the timing and location of protein synthesis. Formation of such granules was emulated by treatment of mouse brain extracts and human cell lysates with a biotinylated isoxazole (b-isox) chemical. Deep sequencing of the associated RNAs revealed an enrichment for mRNAs known to be recruited to neuronal granules used for dendritic transport and localized translation at synapses. Precipitated mRNAs contain extended 30 UTR sequences and an enrichment in binding sites for known granule-associated proteins. Hydrogels composed of the low complexity (LC) sequence domain of FUS recruited and retained the same mRNAs as were selectively precipitated by the b-isox chemical. Phosphorylation of the LC domain of FUS prevented hydrogel retention, offering a conceptual means of dynamic, signal-dependent control of RNA granule assembly.

引用

页码：768 / 779

页数：12

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