N-Alkyl-PEI-Functionalized Iron Oxide Nanoclusters for Efficient siRNA Delivery

被引:101
作者
Liu, Gang [1 ,2 ,3 ]
Xie, Jin [2 ]
Zhang, Fan [2 ]
Wang, Zhiyong [1 ]
Luo, Kui [1 ]
Zhu, Lei [2 ]
Quan, Qimeng [2 ]
Niu, Gang [2 ]
Lee, Seulki [2 ]
Ai, Hua [1 ,4 ]
Chen, Xiaoyuan [2 ]
机构
[1] Sichuan Univ, Natl Engn Res Ctr Biomat, Chengdu 610064, Peoples R China
[2] NIBIB, LOMIN, NIH, Bethesda, MD 20892 USA
[3] N Sichuan Med Coll, Affiliated Hosp, Sichuan Key Lab Med Imaging, Dept Radiol, Nanchong 637007, Peoples R China
[4] Sichuan Univ, Dept Radiol, W China Hosp, Chengdu 610041, Peoples R China
基金
中国国家自然科学基金; 美国国家科学基金会; 美国国家卫生研究院;
关键词
MULTIFUNCTIONAL MAGNETIC NANOPARTICLES; GENE DELIVERY; RNA INTERFERENCE; GOLD NANOPARTICLES; IN-VIVO; POLYETHYLENIMINE PEI; MAMMALIAN-CELLS; BIOMEDICAL APPLICATIONS; CANCER-THERAPY; PLASMID DNA;
D O I
10.1002/smll.201100825
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Small-interfering RNA (siRNA) is an emerging class of therapeutics, which works by regulating the expression of a specific gene involved in disease progression. Despite the promises, effective transport of siRNA with minimal side effects remains a challenge. In this study, a nonviral nanoparticle gene carrier is developed and its efficiency for siRNA delivery and transfection is validated at both in vitro and in vivo levels. Such a nanocarrier, abbreviated as Alkyl-PEI2k-IO, was constructed with a core of iron oxide nanoparticles (IOs) and a shell of alkylated polyethyleneimine of 2000 kDa molecualr weight (Alkyl-PEI2k). It is found to be able to bind with siRNA, resulting in well-dispersed nanoparticles with a controlled clustering structure and narrow size distribution. Electrophoresis studies show that the Alkyl-PEI2k-IOs could retard siRNA completely at N:P ratios (i.e., PEI nitrogen to nucleic acid phosphate) above 10, protect siRNA from enzymatic degradation in serum, and release complexed siRNA efficiently in the presence of polyanionic heparin. The knockdown efficiency of the siRNA-loaded nanocarriers is assessed with 4T1 cells stably expressing luciferase (fluc-4T1) and further, with a fluc-4T1 xenograft model. Significant down-regulation of luciferase is observed, and unlike high-molecular-weight analogues, the Alkyl-PEI2k-coated IOs show good biocompatibility. In conclusion, Alkyl-PEI2k-IOs demonstrate highly efficient delivery of siRNA and an innocuous toxic profile, making it a potential carrier for gene therapy.
引用
收藏
页码:2742 / 2749
页数:8
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